Relationship Between BMD And Histone Demethylase JMJD2 In Patients With Postmenopausal Osteoporosis

ObjectiveAfter the kidney was observed type of postmenopausal osteoporosis bone tissue in patients with histone demethylase JMJD2A, JMJD2B and estrogen receptors ERα, gene and protein expression levels in ERβ and to analyze histone demethylase JMJD2A, correlation JMJD2B and bone mineral density as well as JMJD2A, JMJD2B with ERα, ERβ correlation to discover histone demethylase osteoporosis after contact with the enzyme deficiency type JMJD2 menopause for the treatment of postmenopausal osteoporosis to find new target.MethodsSelect after October 2014 to June 2015 Guangzhou University of Traditional Chinese Medicine First Affiliated Hospital orthopedic admitted because of hip osteoarthritis undergoing total hip arthroplasty 30 cases of postmenopausal female patients, aged 50 to 70 years old preoperative use of dual-energy X-ray absorptiometry was measured preoperative femoral neck bone mineral density, divided into a control group of 15 patients (the amount of normal bone, femoral neck BMD T value>-1.0) and 15 cases of the experimental group (menopause osteoporosis group, femoral neck BMD T value≤-2.5), intraoperative femoral neck surgery take place cancellous bone spare. Application of real-time PCR and Western blot were used to detect cancellous bone tissue expression levels JMJD2A, JMJD2B, ERα and ERβ gene and protein. Experimental data using SPSS20.0 software processing, P<0.05 was considered statistically significant finds. Analysis of correlation JMJD2A, JMJD2B gene and protein expression of bone mineral density using Pearson correlation analysis, correlation analysis JMJD2A JMJD2B with ERα, ERβgene and protein expression between.Results1. Compared with the normal group, the amount of bone in postmenopausal osteoporosis group bone tissue JMJD2A, JMJD2B, ERα and ERβ gene and protein expression levels were significantly decreased, the difference was statistically significant (P<0.01) between the two groups. Compared with normal bone mass in postmenopausal osteoporotic bone tissue OPG mRNA expression levels decreased, RANKL and RANKL/OPG mRNA expression were elevated water, the difference between the two groups was statistically significant (P<0.01)2. Correlation analysis showed that postmenopausal women with BMD positively correlated with JMJD2A, JMJD2B gene and protein expression levels as significant; ERα, ERβ significant positive correlation with JMJD2A, JMJD2B gene and protein expression levels were into.Conclusion1. The group of postmenopausal osteoporosis bone tissue histone demethylase JMJD2A, JMJD2B expression levels were significantly lower than normal bone mass, bone density and JMJD2A, JMJD2B expression levels were positively correlated, suggesting that histone demethylase Enzymes JMJD2A, JMJD2B may be involved in bone metabolism, can reduce post-menopausal osteoporosis occur.2. Two groups of patients bone tissue ERα, ERβ expression levels of histone demethylase JMJD2A, JMJD2B significantly positively correlated, suggesting JMJD2A and JMJD2B possible by regulating estrogen-ER signaling pathway regulates bone metabolism, among them specific transport mechanism remains to be elucidated.3. Histone demethylase JMJD2A, JMJD2B may include kidney deficiency type as a postmenopausal osteoporosis DNase.