Study On Anti-prostatitis And Anti-bph Activity Compositions Of Rape Bee Pollen

The rape bee pollens with irregularly granular shapes are mixtures of the secretion of bee and the pollen of rape flowers, which are created by bees as they gather nectars. It have been proved that the rape bee pollens contain some bioactive materials with functions involving preventing and treating prostatitis, depressing tumors, lowering blood lipids, improving immunity as well as scavenging free radical. As one kind of medicines stemming from plants to treat prostatitis, the curative effect of rape bee pollens is doubtless. However, to reach the curative level, relatively large amount of medicine and long term are required. Moreover, the researches on the mechanisms of rape bee pollens in reducing prostatitis are not complete. Those mentioned above restrict the wide-use of rape bee pollens in curing prostatitis. Therefore, this research focused on the anti-prostatitis bioactive materials extracted from rape bee pollens, which could be considered as four parts:(1)The effect of rape bee pollens extracts on the chromc nonbacterial prostatitis(CNP) was investigated primarily. The bioactive materials were extracted in sequence by 90% ethanol and distilled water. Two portions of extracts from 90% ethanol were further extracted by petroleum ether and acetic ether, respectively. Eventually, four types of extracts in corresponding solvents were obtained. Carrageenan-CNP-Rat model was established to evaluate the anti-CNP bioactivities of the extracts. Results show that ethanol and acetic ether extracts have pronounced effects on reducing the prostate weight, prostate index and interleukin-8(IL-8) in either serum or prostate homogenate(P < 0.05 or P < 0.01), while the bioactivities of water and petroleum ether extracts are less than the other two, which consist with the changes of prostate morphology and histopathology.(2)The bioactive components in ethanol and acid ether were further studied. Both of these extracts were mixed and separated roughly by chromatography with MIC column. The separated components were defined as CI, CII, CIII and CIV and their corresponding anti-CNP bioactivities were tested on rats. Results indicate CII and CIII remarkably reduce the prostate weight, prostate index and interleukin-8(IL-8) in either serum or prostate homogenate(P < 0.05 or P < 0.01), and CIV have a diuretic effect,which are in agreement with the prostate morphology and histopathology.(3)A mixture of CII, CIII and CIV were used to feed androgen-induced benign prostatic hyperplasia(BPH) model rats. It shows a reductive effect not only on the prostate weight, prostate index and IL-8 but also on the concentration of hyperplasia of prostate gland indicators including prostatic acid phosphatase(PAP), testosterone(T) and estradiol(E2). The decrease of IL-8 suggests that benign prostatic hyperplasia might occur with the inflammation.(4)CII, CIII and CIV were separated, respectively, by chromatography with MCI、ODS columns and three chemical compound named as compound-1, compound-2 and compound-3 were achieved. The structures of these compounds were detected by nuclear magnetic resonance(NMR). The structures of compound-1, compound-2 and compound-3 are as follow: Octadecenoic-9Z, 12 Z, 15Z- trienoyl-N- ethyl glyceryl ether;1’-octadecenoic-9Z, 12 Z, 15Z- trien-2’-methoxy-3’-octadecenoic-9Z, 12 Z, 15Z- trienoyl-N- ethyl- glyceryl ether HO-C6H4-CH=CH-CO-NH-(CH2)5-NH2.