The Relationship Between The I/D Polymorphism Of The ACE Gene And Intracerebral Hemorrhage In Yushu Tibetan Patients Of Qinghai

Objective:To analysis the frequency distribution of the insertion/deletion(I/D) polymorphism in the angiotensin converting enzyme(ACE) of Yushu Tibetan intracerebral hemorrhag patient and healthy native, explore the correlation between angiotension converting enzyme(ACE) gene polymorphism and cerebral hemorrhage in Yushu Tibetan patients and healthy subjects of Qinghai.Methods:We collected 52 Tibetan patients(27 males,25 females) with cerebral hemorrhage during the period from February,2015 to February,2016 in Yushu, Qinghai province as case group, and the control group is the 51 cases of healthy subjects(28 males,23 females),who did physical examination in the same period,and their age, gender and place of residence are the same as case group. And collected detailed information of all subjects, including laboratory data. PCR-RFLP method was used to detect genotype of ACE in all samples. ELISA method was used to determine the serum ACE level within 48 hours after the onset of the disease. Finally, all the data were analyzed statistically.Results:1. The sixteenth introns in ACE gene was amplified by PCR, and three bands appeared after electrophoresis: 490 bp bands were homozygous insertion type(II), 190 bp bands were homozygous deletion type(DD), both 490 bp and 190 bp bands were heterozygous insertion / deletion type(ID).2.Two groups were tested by Hardy-Weinberg equilibrium, all of them were consistent with the HWE equilibrium rule(P>0.05). 3. The distribution of ACE genotypes in the case group was as follows: DD was 9 cases(17.31%), II was 21 cases(40.38%), ID was 22 cases(42.31%).The alleles frequency were: D38.46%,I 61.54%; the frequencies of ACE genotypes and the alleles revealed no significant difference between the two groups(genotype: ????0.143,allel:?????0.158,P>0.05). 4. Measured the serum ACE level of 36 cases of patients within 48 hours after the onset of the disease and 36 cases of control group, the difference of the serum ACE level between the two groups were significant(t=2.253,P=0.031).Conclusions: The angiotensin converting enzyme(ACE) gene I/D polymorphism is not related to the ce rebral hemorrhage in Yushu Tibetan patients. The polymorphism of ACE may not be a genetic factor in Yushu Tibetan patients. Tibetan with cerebral hemorrhage had higher serum level of ACE than controls. It is suggested that ACE level may be an index for evaluating the incidence of cerebral hemorrhage.