A Pilot Study Of The Effects Of Lactobacillus Salivarius W22a Against Peri-implantitis Pathogens In Vitro

Objective:This study aimed on the probiotic features of Lactobacillus salivarius w22 a and it’s effects against peri-implantitis pathogens in vitro.In order to provide experimental basis of Lactobacillus salivarius w22 a as probiotics in the prevention and treatment of peri implantitis via the experiments.Methods:1.Preliminary analysis of metabolites of Lactobacillus w22a:Measurement of pH values of metabolites of,using Streptococcus as a control;The content of hydrogen peroxide was detected by semi quantitative test paper;The content of extracellular polysaccharide was measured by phenol sulfuric acid method.2.Measurement of the rate of self polymerization of Lactobacillus w22 a and coaggregation with pathogenic bacteria by using turbidity method.3.To observe the inhibitory effect of Lactobacillus w22a’s metabolites on Pg、Pi、Fn by using the agar diffusion method.4.To observe the tolerance of Lactobacillus salivarius w22 a to lysozyme and antibiotics by using agar diffusion method and co culture method.Results: 1.Preliminary analysis of the fermentation of Lactobacillus salivarius w22 a.Lactobacillus salivarius w22 a could produce organic acid,and acid producing ability was weaker than that of Streptococcus mutants; A certain amount of hydrogen peroxide and extracellular polysaccharide also could be examed.It was suitable for using Lactobacillus salivarius w22 a as probiotics besause of its metabolic characteristics.2.Aggregation experiment showed that Lactobacillus salivarius w22 a got auto-aggregation and co-aggregation abilities with Porphyromonas gingivalis(Pg) 、 Pevotellaintermedia(Pi) 、Fusobacteriumncleatum(Fn);besides,Lactobacillus salivarius w22 a was hydrophobic.The adhesion characteristics might help its colonization in the dental implants environment.3.By applying fermentation of Lactobacillus salivarius w22 a to antibacterial experiments in vitro,it was showed that the fermentation could inhibition the growth of Pg、Pi、Fn in different degrees.It could be concluded that the inhibition was a complex function of many substances in its fermentation.4.The tolerance of antimicrobial substances was evaluated.Lactobacillus salivarius w22 a exhibited tolerance to some kinds of antibiotics.The strain also showed tolerance to 0.8mg/mL Lysozyme solution.It was good for its survival in the oral cavity at some degree.Conclusion: Lactobacillus salivarius w22 a is a strain with potential oral probiotic features and expected to be used as probiotics in the field of prevention and treatment of PI.