Effect Of Angiopoietin-1 On Oxidative Stress In Kidney Of Diabetic Rats

Objective: To evaluate effect of angiopoietin-1 on oxidative stress in kidney of diabetic rats.Methods: Ang-1 adenovirus expression vector was constructed. Rat model of diabetic nephropathy was induced by a single intraperitoneal injection of streptozotocin(55mg/kg).The male SD rats were randomly divided into four groups:(1) Normal control group(NC);(2) Diabetic nephropathy group(DN);(3) Ang-1 intervention group(AV),diabetic rat was treated with Ang-1 adenovirus vector(3×108 pfu per rat) at 8 weeks via penile vein;(4) Blank adenovirus group(BV), diabetic rat was treated with blank adenovirus vector(3×108 pfu per rat) at 8 weeks via penile vein. In the end of 12 and 16 weeks, serum creatinine, urea and renal histopathological changes were measured. The levels of superoxide dismutase(SOD), malondialdehyde(MDA), advanced oxidation protein products(AOPP), and advanced glycation end products(AGE) in renal tissue were respectively detected by Xanthine oxidase method, Thiobarbituric acid method,Enzyme-linked immunosorbent adsorption assay(ELISA).Results:(1) Blood and urine routine index: The levels of blood glucose and urine protein in three diabetic nephropathy model groups were significantly higher than those of the NC group(P<0.05), and there were no differences in the three model groups(P>0.05). The blood glucose in AV group and urine protein in DN and BV groups were significantly higher at 16 W than 12W(P<0.05). Serum creatinine had no significant difference between four groups(P>0.05), but its level in AV group was higher at 16 W than 12W(P<0.05).Serum urea in AV group was higher than that of NC and DN groups(P<0.05).(2) Renal tissue pathology: Increased kidney size, kidney weight index, glomerular volume, and mesangial matrix were found with dilation of partial renal tubule and epithelial vacuolar degeneration in three diabetic model groups. These pathological changes in AV group were relatively reduced.(3) Oxidative stress index: The level of SOD in renal tissues of three diabetic nephropathy model groups was significantly lower than that of the NC group(P<0.05), and its level in AV group was significantly higher than that of DN and BV groups at12W(P<0.05). SOD level in DN and BV groups was significantly higher at 16 W han 12W(P<0.05). The levels of MDA, AOPP, and AGE in renal tissues of three diabetic nephropathy model groups were significantly higher than those of the NC group(P<0.05),but the above indices in AV group were lower than those of DN and BV groups at 12W(P<0.05). MDA, AOPP, and AGE levels in renal tissues of DN and BV groups were significantly lower at 16 W than 12W(P<0.05). But there no significant difference was found between 16 W and 12 W in AV group(P>0.05).Conclusions: Ang-1 can improve the oxidative stress state of diabetic kidney.