| Objective : To investigate the relationship between the polymorphisms of blood coagulation factor V Leiden(rs1799963) 、 prothrombin G20210A(rs6025) and thrombomodulin gene c.1418C>T(rs1042579),age,the clinical biochemica l indexes of triglyceride(TG)、cholesterol(C HOL)、low density lipoprotein(LDL)、high density lipoprotein(HDL)、D-dimer(DD)、fibrinogen(FIB)、fibrinogen degradation product(FDP) and homocysteine(Hcy) and the lower extremity deep venous thrombosis.Methods:This study chose 157 cases who were diagnosed as the lower extremity deep venous thrombosis from November 2014 to November 2015 as case group,164 cases of healthy people who were excluded from thrombotic diseases、malignant tumor、fracture、pregnancy、autoimmune diseases and blood system diseases as control group. All subjects of the case-control study were collected the basic data of age、the clinical biochemical indexes of triglyceride、cholesterol、low density lipoprotein、high density lipoprotein、D-dimer、fibrinogen、fibrinogen degradation product and homocysteine;O ur study detected the genotypes of all objects through the technology of KASP, containing the sites of rs1799963、rs6025 and rs1042579;By chi-square test and Logistic regression analysis,this paper discussed the relationships with the lower extremity deep venous thrombosis.Results:(1)The average age of case group was 55.32±14.65 years old,under the age of 40 was 25 people(15.9%),above 40 years old were 132 people(84.1%);The average age of case group was 42.70±16.40 years old,under the age of 40 was 67 people(40.9%),above 40 years old was 97 people(59.1%);The age distribution had significant differences between two groups( χ2=24.382, P < 0.05);( 2) The TG of case group was 1.5±0.80mmol/L,control group was 1.62±1.70mmol/L,there were significant differences between two groups(P<0.05);The TG of case group was 1.5±0.80mmol/L,control group was 1.62±1.70mmol/L,there were significant differences between two groups(P<0.05);The CHO L of case group was 4.09±1.58mmol/L,control group was 4.76±0.81mmol/L,there were significant differences between two groups(P<0.05);The LDL of case group was 2.43±1.21mmol/L,control group was 2.87±0.88mmol/L, there were significant differences between two groups( P < 0.05); The HDL of case group was 1.05±0.31mmol/L, control group was 1.02±0.48mmol/L, there was no significant difference between two groups(P>0.05);The DD of case group was 671±824ng/ml,control group was 127.50±152ng/ml,there were significant differences between two groups(P<0.05);The FIB of case group was 4.24±2.30g/L,control group was 2.49±0.63g/L,there were significant differences between two groups(P<0.05);The FDP of case group was 7.05±16ug/L,control group was 1.07±1.37ug/L,there were significant differences between two groups( P < 0.05); The Hcy of case group was 17.43±8.14umol/L, control group was 11.56±3.36umol/L, there were significant differences between two groups(P<0.05);(3)According to the maximum of the clinical reference values of TG、CHO L、LDL、HDL、DD、FIB、FDP and Hcy for the boundary value,our study divided all the research subjects into two part,the chi-square test showed that,in the index of TG(the clinical reference value is 0.56-1.7mmol/L),the groups between TG≤1.7mmol/L and TG>1.7mmol/L had no significant difference(χ2=2.454,P>0.05);in the index of CHOL(the clinical reference value is 3.1-5.7mmol/L),the groups between CHOL≤5.7mmol/L and CHOL > 5.7mmol/L had significant differences(χ2=5.801,P<0.05);in the index of LDL(the clinical reference value is 0-3.12mmol/L),the groups between LDL≤3.12mmol/L and LDL>3.12mmol/L had significant differences( χ2=5.359, P < 0.05); in the index of HDL(the clinical reference value is 0.93-1.93mmol/L),the groups between HDL≤1.93mmol/L and HDL>1.93mmol/L had no significant difference(χ2=0.606,P>0.05);in the index of DD(the clinical reference value is 0-255ng/ml),the groups between DD≤255ng/ml and DD>255ng/ml had significant differences(χ2=156.302,P<0.05);in the index of FIB(the clinical reference value is 1.69-5.15g/L), the groups between FIB≤5.15g/L and FI B > 5.15g/L had significant differences(χ2=58.956,P<0.05);in the index of FDP(the clinical reference value is 0-2.01ug/L),the groups between FDP≤2.01ug/L and FDP >2.01ug/L had significant differences(χ2=138.763,P<0.05);in the index of Hcy(the clinical reference value is 5-15umol/L),the groups between Hcy≤15umol/L and Hcy>15umol/L had significant differences(χ2=64.357,P<0.05);(4)The results of rs1799963 polymorphism showed that,in case group,GG genotype had 156 cases,GA genotype had 1 case,AA genotype had 0 case;in control group,our study had GG genotype of 164 cases without other genotypes. The results of polymorphism of rs6025 showed that,it only had GG genotype of 157 cases in case group and GG genotype of 164 cases in control group. In case group,the distribution frequency of rs1042579 were CC genotype of 69 cases(43.9%)、CT genotype of 76 cases(48.4%) and TT genotype of 12 cases(7.7%),the frequency of allele C was 70.1%,the frequency of allele C was 29.9%;In control group,the distribution frequency of rs1042579 were CC genotype of 97 cases(59.1%)、CT genotype of 62 cases(37.8%) and TT genotype of 5 cases(3%),the frequency of allele C was 78.0%,the frequency of allele C was 22.0%. The results of chi-square test showed that,the distribution of each genotype frequency had significant differences(P<0.05);The frequency of C T+TT genotype and the distribution of allele frequency had significant differences(P<0.05);(5)The analyses of Logistic regression showed age(OR=2.340,95%CI 1.017~5.381,P<0.05)、DD(OR=16.560,95%CI 7.182~38.185,P<0.001)、FDP(OR=9.983, 95%CI 4.556~21.876, P < 0.001) 、 Hcy( OR=9.872, 95%CI 4.194~23.236,P<0.001)、the genotype CT+TT of rs1042579(OR=2.151,95%CI 1.010~4.579,P<0.001).Conclusion:(1)The ploymorphsims of FV Leiden(rs1799963)and prothrombin G20210A (rs6025)had no relationship with lower extremity deep venous thrombosis;(2)Allele T of rs1042579 may be a risk factor for the lower extremity deep venous thrombosis;(3)The independent factors were age、D-dimer、FDP、Hcy and the CT+TT genotypes of rs1042579 for the lower extremity deep venous thrombosis in our study. |
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