Study On The Effection Of Extracellular Histones On Intestinal Mucosa Barrier Function

Objective:To investigate the effect and intervention value of mouse intestinal mucosal barrier by extracellular histones.Methods:20 healthy male C57BL/6 mice were include in this research and divided into 2 groups randomly:10 in control group and 10 in experimental group. Animals in the experimental group were injected extracellular histones with the concentration of 50mg/kg into mice via the tail vein. Animals in control group were injected with equivalent normal saline via the tail vein. After 3 hours, collected the blood sample and intestinal tissues and observed the intestinal mucosa histomorphology by light microscopy and TEM. The expression quantity of tight junction protein (ZO-1, occludin, Claudin-1) were detected by western blot. The content of diamine oxidase (DAO) and intestinal fatty acid binding protein (I-FABP) were detected by ELISA and the plasma endotoxin was determined by Limulus test.Results:Compared with the control group, the intestinal pathology of experimental group was significantly damaged. The tight junction was damaged, the expression quantity of tight junction protein was decreased and the contents of DAO and I-FABP and the endotoxin level were significantly increased.Conclusion:The extracellular histone undermined the integrity of mouse intestinal mucosal barrier, which was an important factor to cause sepsis. The extracellular histones-targeted intervention may be a new strategy for the treatment of sepsis and is worthy of further exploration.