Effects Of Long-term Aerobic Exercise On PI3K/GSK-3Signal Transduction Pathway In The Liver Insulin Of Obese Rats

Objective：This experiment adopts high-fat diet-induced obese rats model to observe the influenceendurance exercise of8weeks have on liver insulin PI3K/Akt/GSK-3signal pathways ofobese rats. This experiment aims to discuss molecular regulatory mechanism of aerobicexercise for improving insulin resistance.Research methods：Randomly select10rats as the control group (CON) and the remaining38rats ashigh-fat diet group from4815-month-old male SD rats. After8weeks’ high-fat diet, get rid of6obesity-resistant rats and take out30top obese rats from the remaining32obese ratsaccording to body weight from high to low, and then randomly divide them into three groups:group for10rats on high-fat diet (HFD), continuous exercise group1for10rats on high-fatdiet (HFD+CE1), and continuous exercise group2for10rats on high-fat diet (HFD+CE2).Then provide eight weeks’ swimming training for rats in HFD+CE1group and HFD+CE2group. At the end of the swimming training, take rats in each group for sampling to determinethe content of blood glucose, blood lipid, insulin, glycogen, liver triglyceride (TG), free fattyacid（FFA） and protein expression and phosphorylation levels of liver PI3K/Akt/GSK-3.Research results：1. When sampling, for rats in HFD group, their body weight, abdominal fat content, fastinginsulin and its resistant index, serum cholesterol(TC), triglyceride (TG), low-densitylipoprotein protein (LDL-C) for sampling are significantly higher than that of CON group,（P<0.05,P<0.05，P<0.05，P<0.05，P<0.01，P<0.01，P<0.01）, high density lipoprotein(HDL-C), and liver glycogen significantly decreases（P<0.01，P<0.05）, liver free fatty acid (FFA) and TG are significantly higher than that of CON group（P<0.01，P<0.01）. There is nochange for blood glucose, and protein expression of liver PI3K-p85and GSK-3βissignificantly higher than that of CON group（P<0.01，P<0.05）. Akt2protein expression andphosphorylation level are significantly lower than that of CON group（P<0.05，P<0.01）andGSK-3βphosphorylation expression shows no significant difference（P>0.05）.2. After8weeks’ exercise, for rats in HFD+CE1/2group, their body weight、abdominal fatcontent、fasting insulin and its resistant index are significantly lower than that of HFD group（P<0.05）. For rats in HFD+CE1/2group, their glycogen, TG and FFA are significantlyhigher than that of HFD group（P<0.05，P<0.05，P<0.01）,serum TG and LDL-C are lowerthan that of HFD group（P<0.01，P<0.01）, and HDL-C is higher than that of HFD group（P<0.01）while blood glucose and TC shows no significant difference; protein expressionof liver PI3K-p85and GSK-3β of rats in HFD+CE1/2group significantly decreases（P<0.01，P<0.05）, Akt2protein expression and phosphorylation level of rats in HFD+CE1/2group aresignificantly higher than that of HFD group（P<0.05，P<0.05）,and GSK-3βphosphorylationlevel of rats in HFD+CE1/2group significantly increases.3. There is no significant difference except that liver PI3K-p85of rats in HFD+CE2group issignificantly higher than that of HFD+CE1group（P<0.05）.Conclusions：1. Long-term high-fat diet-induced rat liver PI3K/GSK-3signal the impaired transductionpathways, the body hepatic lipid disorders;2. Aerobic exercise can repair liver insulin PI3K/GSK-3signal transduction pathways ofobese rats to alleviate liver insulin resistance and improve the body’s fat and carbohydrate metabolism disorders. The after effect of aerobic exercise can continue for48hours.