Application Of DNA Sub-tile Self-assembly For Logic Operations And Constructing Three-dimensional Structures

As the genetic material, DNA is considered to be a kind of perfect nanotechnology material, which almost carried all of the genetic information. It not only has exquisite, flexible,and stable structure, but also is a nano-material with assembly and encoding capabilities.DNA nanotechnology is a kind of high technology with broad prospects, which put DNA as a kind of nano-material and fuse with the nano technology well. After thirty years of development, DNA nanotechnology had become a hot research subject and made great achievements in the field of molecular computing, nano-medicine, molecular biosensors, and biometric, etc.With the fundamental and essential knowledge of DNA sub-tile, in this paper we explore this self-assembly method in logical operation and building three-dimensional structures.Firstly, this paper propose an algorithm self-assembly model by DNA sub-tile asymmetric design ideas and full addressability, which based on DNA sub-tile self-assembly.Assembling 4-arm tiles, then depending on the level-2 sticky-ends for a specific connection between the tiles, the array complete the XOR and XNOR logic operations. And then we design half adder based on the theory above. This programme make a theoretical exploration to building DNA molecular logic circuit, which combine DNA computing with DNA nano technology.Secondly, we use flexibility of DNA sub-tile in two- and three-dimensional space to build three-dimensional structures. Then modify the length of DNA sub-tile, and put forward to construct regular tetrahedron inscribed cube. We use NUPACK to design and analysis DNA sequence of tetrahedron, and through specific biochemical experiments assembled DNA tetrahedron. At the same time, a method using concentration to control tetrahedral structure has been proposed. This provides a new method for the construction of complex nanometer spatial structure.Finally, summarized the primary coverage of this article and made a promising prospect for the future research direction.