Fermentation Of Ulva Lactuca And Effects Of Fermentation Broth On Spirulina Maxima

Our country is rich in resource of Ulva pertusac, but it's barely be harvested in time, and the most important problem is how to fully use the salvaged Ulva pertusa. This paper mainly studied the optimizing condition of acid hydrolysis on Ulva pertusa, the manufacture of Ulva pertusa fermented broth, the influence of Ulva pertusa fermented broth to the growth of Spirulina platnsis and the application of fermentation broth in simplified medium. The main results are as follows:1. To optimize the condition of acid hydrolysis on Ulva pertusaIn this experiment, Ulva pertusa was treated by diluted solution of sulfuric acid. Through single test of diluted acid concentration, hydrolysis temperature, hydrolysis time and solid-to-solution ratio, the treatment conditions by four-factor and three-level orthogonal test was optimized. The impacts of four factors from top to bottom are diluted acid concentration, solid-to-solution ratio, hydrolysis temperature, hydrolysis time. The best conditions are as follows:diluted acid concentration of 1%(v/v), solid-to-solution ratio was 1:10(w/v), hydrolysis temperature at 90?, and hydrolysis time was 70min.2. The manufacture of Ulva pertusa fermented brothThe biochemical component of fermented broth has been increased significantly after fermented by Saccharomyces cerevisiae and Bacillus subtilis. After fermentation of Saccharomyces cerevisiae, the reducing sugar is up to 45.7%, the organic matter is up to 34.2%, the protein is increased by 74.3%, the amino acid is increased by 52.2%. After fermentation of Bacillus subtilis, the reducing sugar is up to 40.4%, the organic matter is up to 48.7%, the protein is increased by 22%, the amino acid is increased by 36.2%.3. The influence of Ulva pertusa fermented broth on the growth of Spirulina platnsiThere was promoting effect on the growth of Spirulina platnsis after adding two kinds of fermented broth. The affection of Ulva pertusa (Saccharomyces cerevisiae) fermented broth was highly significant (P<0.01), and the affection of Ulva pertusa (Bacillus subtilis) fermented broth was significant (P<0.05). When the addition was 1%, the max growth rate was 32.31%(Saccharomyces cerevisiae),31.69%(Bacillus subtilis); and the growth rate of control group was 28.1%(Saccharomyces cerevisiae),27.86% (Bacillus subtilis).4. The growth of Spirulina platnsis in simplified mediumMaking three kinds of simplified medium with a small amount of fermented broth: 3/4(group one), 1/2(group two), 1/4(group three). The growth of Spirulina platnsis in part of the simplified medium is better than those in normal medium; especially the addition is 1%. There is also a small increase about the content of pigment and protein.