Chemical Constituents Of Talaromyces Wortmannii LGT-4 Isolated From Tripterygium Wilfordii And Colletotrichum Gloeosporioides GT-7 Isolated Form Uncaria Rhynchophylla

In this paper,two new pyranone and twelve known compounds were isolated and identified from a strain of Talaromyces wortmannii LGT-4,a endophytic fungus of traditional Chinese medicinal plant Tripterygium Wilfordii and Colletotrichum gloeosporioides GT-7,a endophytic fungus of Uncaria rhynchophylla by using modern chromatographic methods,including silica gel column chromatography,MCI column chromatography,Sephadex,LH-20,HPLC etc,and spectrum methods such as NMR,IR,ESIMS,2DNMR,ECD etc.The antiacetylcholinesterase and monoamine oxidase inhibitory activities of these compounds were tested by using 96-well micro-plate methods.The main results were as following:1.Two new pyranone and five known compounds were isolated from ethyl acetate extract of T.wortmannii LGT-4.Two new pyranone were named as Wortmannine F?1?and Wortmannine G?2?,and five known compounds were identified as Cyclo?L-Leucyl-L-Prolyl??4?,cyclo[L-?4-hydroxyprolinyl?-D-leucine]?5?,cis-cyclo?L-Ala-L-Pro??6?,tryptamine?7?,cyclo?D-Pro-L-Leu??8?.2.Seven known compounds were isolated from ethyl acetate extract of C.gloeosporioides GT-7.These compounds were identified as 14-Epi-14-hydroxy-10,23-dihydro-24,25-dehydroaflavinine?9?,10,23-Dihydro-24,25-dehydro-21–oxo-aflavinine?10?,Ergosterol?11?,Ergosterol peroxide?12?,Clavatustide C?13?,Chrysosporide?14?,Ursolic acid?15?.3.All of the compounds were tested for their monoamine oxidase?MAO?and acetylcholinesterase?AChE?inhibitory activities.The results showed that only compound 14 had weak inhibitory activity against AchE with IC₅₀0 of 24.2?g/mL.