Toxicity And Toxicology Studies On Heavy Metals (Cr⁶⁺,Ni²⁺,As³⁺) In Juvenile Pseudosciaena Crocea

In June 2016,in the Xi Xuan breeding test field of Zhejiang Ocean Fisheries Research Institute selected day old 103 d of artificial cultivation of juvenile Pseudosciaena crocea as experimental animals,the hydrostatic method to carry out research Cr⁶⁺,Ni²⁺ and As³⁺ on juvenile P.acute toxicity experiment.The results showed that 96 h LC50 values of Cr⁶⁺,Ni²⁺ and As³⁺ towards P.in the sea water with condition of pH 7.86⁸.12,a temperature of 23.1²4.7?,and a salinity of 27.6²9.1 were 10.262 mg/L,46.221 mg/L and 9.134 mg/L respectively.96 h LC50 values with 95% confidence interval were in the range of 6.676¹2.672 mg/L,42.942⁴9.827 mg/L and 6.449¹1.272 mg/L,respectively.The acute toxicity of Cr⁶⁺,Ni²⁺ and As³⁺ to P.was arranged in a descend order as the following: As³⁺>Cr⁶⁺>Ni²⁺.The safe concentration of Cr⁶⁺,Ni²⁺ and As³⁺ on juvenile P.were 0.10 mg/L,0.46 mg/L,0.09 mg/L.The toxicity of chromium,nickel and arsenic on juvenile P.were highly toxic.According to the results of experiments on acute toxicity of juvenile P.Cr⁶⁺,Ni²⁺,As³⁺,the hydrostatic method to carry out research Cr⁶⁺,Ni²⁺ and As³⁺ on juvenile P.acute toxicity exposure experiment,the concentration of 1/10 96hLC50,which were 1.026 mg/L,4.622 mg/L,0.913 mg/L.On the 24,48,72 and 96 h,studied Cr⁶⁺,Ni²⁺ and As³⁺ had an impact on brain,liver,gill,muscle tissue of P.about SOD,CAT,GSH-PX and AChE.The results showed the activities of SOD,CAT,GSH-PX and AChE in the control group were normal in the concentration of 1/10 96hLC50 of Cr⁶⁺,Ni²⁺ and As³⁺,which were 1.026 mg/L,4.622 mg/L,0.913 mg/L.The activities of SOD,CAT,GSH-PX and AChE in the tissues of Cr⁶⁺,Ni²⁺ and As³⁺ were significantly changed?p < 0.05?and in 96 h,the organization showed different trends,showing the corresponding induction and inhibition.