Design, Synthesis And Protein Recogintion Of Glucose Based Polymer Modified Gold Nanoparticles

In recent years,gold particles have fluorescence properties,supramolecular,protein recognition and other special physical.Carrying nucleic acids and carbohydrate macromolecules for immunization,calibration,and tracking areas.Because of its excellent biocompatibility and easy control of the morphology and structure,the multifunctional copolymer micelles have been used as a new type of polymeric materials.The micelles of this kind of multifunctional copolymers are loaded onto gold nanoparticles and have gradually become a hot spot in biomedical research.Based on a large number of previous work,the gold particles prepared by the one-pot method.Itare designed and synthesized by Reversible Addition-fragmentation chain transfer(RAFT).The chemical structure of the molecules is confirmed by various characterization methods.The self-assembly behavior of the copolymers is obtained by intermolecular interaction force.The function in temperature response,biocompatibility,specific recognition of proteins and its function in inducing apoptosis of hepatocarcinoma cells is studied systematically.In the chapter 1,the research status of gold nanoparticles and the synthesis methods of gold micelles.Thermosensitive glycoconjugates and their applications in biology.The background of the study is briefly introduced.In the chapter 2,the main contents are the synthesis of a series of thermosensitive carbohydrates and thermoresponsive glycopolymers@AuNPs using RAFT polymerization and "one-pot" design.The chemical structures are characterized by technical methods.In order to obtain the copolymer @AuNPs with biological activity and temperature sensitivity,6-O-vinyl azelaic acid-D-glucose ester(OVNGlu)is prepared by enzymatic method.(OVNGlu)and temperature-sensitive monomer dimethy lene glycol monomethyl ether methacrylate(DEGMA)are synthesized by the RAFT method.P(DEGMA-co-OVNGlu)and PDEGMA-b-POVNGlu with different structures.Then,P(DEGMA-co-OVNGlu)@AuNPs and PDEGMA-b-POVNGlu @AuNPs synthesized by "one-pot" method.The structures of the polymers are characterized by ~1H NMR,FT-IR,UV-Vis and GPC.The results showed that the chemical composition of thermosensitive polymer is consistent with the experimental design.The molecular weight of the polymer can be effectively controlled by RAFT polymerization.The structure-regulated thermosensitive polymer@AuNPs can be prepared by RAFT polymerization and "one-pot" technique.Chapter 3,thermoresponsive sugar-containing polymers with different structures and their micellar self-assembly mechanism are investigated in detail.The low critical solution temperature(LCST)of thermoresponsive glycopolymers,thermoresponsive glycopolymers@AuNPs.The copolymer P(DEGMA-co-OVNGlu)is investigated by UV-Vis spectrophotometer.The LCST of the P(DEGMA-co-OVNGlu)is 34 °C,and the LCST value of the PDEGMA-b-OVNGlu is 35 °C.The LCST of the block copolymer PDEGMA-b-OVNGlu is 36 °C and the LCST of PDEGMA-b-OVNGlu@AuNPs is 37 °C.The results show that the LCST of thermoresponsive glycopolymers@AuNPs is higher than thermoresponsive glycopolymers.To obtain average hydrodynamic radius(Rh)and average gyration radius(Rg)at different temperature,the Laser light scattering(LLS)is used combined static laser scattering(SLS)and dynamic laser scattering(DLS).Rh and Rg of thermoresponsive glycopolymer@AuNPs micelles are depended on the temperature,and the coil-globule transition also is revealed.Micelle morphology of the thermoresponsive glycopolymer@AuNPs micelles is investigated by transmission electron microscopy(TEM),which showed self-assembled micelles with uniform sizes and a regularly spherical shape.The result revealed that thermoresponsive glycopolymers@AuNPs prepared by RAFT polymerization and "one-pot" technique are able to self-assemble into globose nano-micelles,and the LCST values of glycopolymers could be adjusted precisely.According to this study,biomedical polymer system and application of glycopolymers are enriched.In the chapter 4,we aimed at thermoresponsive glycopolymer@AuNPs micelles fabricated by self-assembling to study the structure effect on protein recognition capacity,the temperature influence on protein recognition capacity,the ability of targeted combination between micelles and two different kinds of cells.The results showed that the structured thermogravimetry block copolymers@AuNPs micelles had higher recognition efficiency than ungrafted gold nanoparticles.As the temperature increases,more glycosyl groups are exposed to the surface of the molecule.So that the polymer micelles recognition lectin is better.All the synthesized polymers have good biocompatibility to normal cells.The polymer@AuNPs micelles can recognition lectin and then inhibit the hepatocellular carcinoma cells grouth.In the chapter 5,we summaried the main content in this study,and also look into the future in this field.