The Detecting Technology Of QPCR For The Adulterated Sesame Oil And Peanut Oil

With the rising of the living standard,the consumption of the edible oil is increasing everyday,the increase of the demand promote the development of edible oil industry and economic growth at the same time,However this strong market adds the ambition of criminals to seek profits.Recently,the adulteration of edible oil emerges in an endless stream.It has seriously damaged the legitimate rights and interests of consumers,and posed a threat to their health.But there is no one method which is stable and accurate for detecting the adulterate edible oil.In this study,we used technology of conventional PCR and qPCR,the peanut oil and the sesame oil from different brand oil choosed from market was used as samples,while soybean oil as the adulteration oil.We studied optimization method in DNA extraction,qualitative and quantitative methods from plant oils.Analysis results of this study include as following aspects:(1)We Screened the gene-specific primers of sesame、peanut and soybean through gradient temperatures of PCR;(2)We optimized a plant DNA extraction kit,which was one better method of extracting DNA from plant oil for quality and quantity by means of comparing artificial extracting method with extraction kit method with high efficiency of extraction;(3)This study established a method of the qualitative test according to the specificity of Tm(Melting temperature)value of qPCR product.(4)We accomplished the establishment of standard curve of concentration gradient and the determination for adulteration degree of unknown sample by five times gradient dilution the DNA of vegetable oil.