Regioselective Synthesis And Application Of The Chiralstationary Phases Based On Polysaccharide Derivatives For HPLC

As the increasing of quality of medicine and food, people are paying more attention to the safety of pharmaceutical and food. Therefore, purification and separation of medicine and food has become a big problem today. The emergence of high performance liquid chromatography (HPLC) has greatly contributed to the analysis and separation of chirality compound. HPLC has many especially characteristics, such as rapid, accurate and will not cause damage of both physiological activity and structure of enatiomers. It has become a wide chromatographic separation technique used in the field of medicine, food and so on.In HPLC,chiral stationary phase (CSP) is the core technology for chromatograph separation. And in a wide range of chiral stationary phase, polysaccharide CSPs is undoubtedly the most widely used which has the best ability for chromatograph separation.In this study, based on the regional selection, phenyl isocyanate, 3,5-dichlorobenzene isocyanate had been connected to 2-,3- position. And 11-bromoundecanoic acid, 6-bromine caproic acid and triphenylchloromethane were connected to 6- position. Then through azide reaction, the new derivative of polysaccharide with fullerene has been producted succefully.Comparing the nine polysaccharide derivatives by their mian chain structure and the different substituent in 2-,3-,6- position to research the different of chiral separation ability. In addition,I also discusse the different chiral separation ability in different mobile phase.Polysaccharidederivatives CSPs were packed to the stainless steel columnby slurry method.The derivatives were coated to ammonia propyl silicone, then hexane and isopropanol as the mobile phase. The nine CSPs were evaluated by HPLC.As the result, -Cl as the electron-withdrrawing group could increase ability of chiral separation. And with the neat and flexible groups in 6- position, it could increase the ability of chiral separation. About the fullerene, though it increase the ?-? reaction, the space steric hindrance of fullerene molecules led to the decrease of the chiral separation ability.