Research Of 1-Methylcyclopropene On Grape Texture Under Different Temperature

1-MCP is a type of ethylene receptor inhibitor found in recent years, which can inhibits the induction and fruit ripening related a series of physiological and biochemical reactions.It was also shows that 1-MCP could inhibit the ethylene production, delay the fruit texture worsing and maintain fruit quality in non-respiratory variant fruit. However, some studies had shown that 1-MCP treatment has little effect on quality in non-breathing jump fruit. In this study, 1-MCP was grape preservative to analyze physiological and biochemical indexes and texture characteristics of Kyoho grape under different storage temperature. The use of GC-MS, GC, physical structure of the instrument to analyze the adsorption and diffusion characteristics of 1-MCP on different organs of grape and the effect of 1-MCP on grape surface wax. The mechanism of action of 1-MCP and the softening mechanism of grape were studied.The quality of Kyoho grape berries with different storage conditions and treatment was tested by physiological and biochemical experiments, which indicated that 1 - MCP treatment could maintain the fruit quality at early stage in 0℃ room, but the effect was not obvious. There was no significant difference between 1-MCP treatment and control in cool room. 1-MCP treatment could maintain the fruit quality at late stage in 20℃ room, and the effect was obvious, which especially could matntain VC content, titratable acid content,water content and inhibit weight loss rate, decay rate.Physiological and biochemical experiment were tested related softening enzymes in Kyoho grape berries with different storage conditions and treatment. It was also shows that 1-MCP treatment could significantly inhibit the activities of PG and Cx in Kyoho grape berry, maintaining cellulase content.The results indicated that The results indicated that the the reactive location of 1-MCP is the stratum corneum and the cell wall of each cell.The use of GC to analyze 1-MCP adsorption site.The results showed that the adsorption rate of 1-MCP on the peel of Kyoho grape was higher than pulp. the adsorption capacity of 1-MCP on the peel of Kyoho grape was higher than pulp. The results indicated that the the reactive location of 1-MCP is pericarpThe waxy structure and composition of the surface of Kyoho grapevine were analyzed by GC-MS and scanning electron microscopy. The results showed that the main components of Kyoho grape wax lamina crystals were Octadecanal, Octadecanoic acid ethyl ester and Heptadecanoic acid ethyl ester. The main components of wax film may be Heptacosane and Nonacosane. With the increase of storage time, the number of lamellar crystals has been decreasing and its volume has been getting smaller in 20℃ room. 1-MCP can inhibit the reduction of lamellar crystals and its volume. The results indicated that The results indicated that the the reactive location of 1 -MCP is pericarp.4400 grape berries were analyzed by puncturing test and TPA test. The result of puncture test showed that 1-MCP treatment and control had the greatest effect on skin hardness and skin stiffness, which indicated that the the reactive location of 1-MCP is pericarp . The fruit shape had no effect on the puncture parameters. The results of TPA showed that there was no significant difference in the TPA texture parameters between 1-MCP treatment and control treatment, but 1-MCP treatment had the greatest impact on elasticity, cohesion and chewiness. Fruit shape had effect on TPA parameters.