| Hypochlorite plays an indispensable role in our daily life.It is widely used as a bleacher,a disinfectant and a deodorant in family,hospital,hotel,food processing and so on.It can make our lift healthy,safe and convenient.But when hypochlorite as an efficient chlorinated disinfectant is used,this process will produce chlorate or chlorite,which has potential hazards to the human body.In the biological system,hypochlorous acid and its conjugate base hypochlorite are one of particularly significant reactive oxygen species,which play a very unique function in physiological as well as pathological aspects.In biological system,the endogenous hypochlorite can be produced by peroxidation of chloride ions catalyzed the enzyme myeloperoxidase.Due to their strong oxidizing,they can control and mediate a wide range of physiological and biological process,such as sterilization,disinfection,control of cell life cycle,enhance the antigen immunity,reparation of wound and tissue regeneration.However,various researches have shown that the abnormal concentration of hypochlorite in the living body is closely associated with various diseases,such as neurodegenerative disease,atherosclerosis,cancer,ischemia-reperfusion injury,rheumatism and rheumatoid diseases.Therefore,it is very necessary to detect hypochlorite in the daily life or physical system.And the fluorescent molecular probe is a kind of efficient,convenient and sensitive method for detecting hypochlorite,which has been widely accepted by researchers in recent years and has become a hotspot in chemical and biological fields.In our work,four turn-on ClO~-fluorescent probes were designed.The recognition responses between probes and ClO~-were investigated by fluorescence and UV-Vis spectra.The experiments indicated that all the probes had good selectivity,high sensitivity,low detection limit and fast respond time towards ClO~-.In addition,these interesting recognition mechanisms were confirmed by NMR and MS methods,which will contribute to the further study on the mechanism of ClO~-induced diseases in vivo.Detailed as follows:1.Two commercial compounds as ClO~-fluorescent probes 1 and 2 were designed.And their spectral properties were studied by fluorescence and UV-Vis spectra.Two probes had good selectivity and sensitivity.Commercia l compounds can be direct used for the detection,make up the deficiencies of traditional chemical probe,such as,the complex synthesis process,consuming time and so on.2.1,8-naphthalimide derivatives had high fluorescence quantum yield,large Stokes shift,good light stability and easy modification.Therefore,we choose 1,8-naphthalimide as a fluorophore to synthesize two analogues 3 and 4 for the detection of ClO~-.The results showed that both two probes had good responses towords ClO~-.When ClO~-was added,the fluorescence intensity of probe 3 and 4 was enhanced about 42 and 95 times at 515 and 528 nm,respectively.In addition,the probe 3 was used for fluorescence imaging in living A549 cell.Also,they were applied for detecting ClO~-in water samples.In a word,the experiments of four Turn-On fluorescent probes showed that they all had good responses to ClO~-.Compared to the first two commercially available compounds,water soluble and spectral properties of the latter two probes were improved.More importantly,the probe 3 could apply for imaging ClO~-in living A549 cell.Also,both probe 3 and 4 were used for detecting ClO~-in water samples. |
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