MBL Gene Polymorphisms With Association Of Susceptibility To Tuberculosis

Objective: A case match control study were carried out to aim at exploring therelationship between the polymorphisms of MBLgene (rs1800450G/A) and the susceptibilityto pulmonary tuberculosis (PTB) among the populations in Ningxia Hui Autonomous Region(NHAR), P.R. of China.Methods: All the confirmed PTB cases, who had been diagnosed according to WHOTB-diagnosis criteria, without any other co-infectious condition, in different local TB clinicsin NHAR, were recruited under free-will in2010, and also the healthy match controls, whowere selected by the criteria of the same sex, nationality, similar age and residential area tothose cases but without any health condition. All the participants were interviewed by TBclinic staff with a questionnaire sheet regarding to the demographic information and thefactors related to the diagnosis, treatment and TB transmission risks. The participants wererequired2-5ml venous blood in EDTA tube, then stored at-20oC within30minutes postsampling before DNA extraction. After the genomic DNA extraction, the genotyping of theMBL gene and the detection of the SNP in rs1800450was carried out by use of thepolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The dataof those questionnaires and the genotyping were entered into the SAS9.1software package.After the effective value of population SNP data testing by use of Hardy-Weinberg Lawmethod, a multiple logistic regression analysis (MLR) were used for the selecting the value ofSNP frequencies in different gene presentation models of the different population groups being sort by the demographic information between PTB cases and healthy controls. Thesignificant difference was determined in95%confidential intervals with P <0.05.Results: The distributions of the SNP on MBL gene among the900PTB cases and870healthmatch controls were assessed by value (P>0.05) in local populations. genotype rs1800450allele G, A gene frequencies and genotypes GG, GA, AA were significant differencesbetween cases and controls, recessive model in carrying the AA genotype the incidence ratethan individuals carrying GG and GA individuals tended to increase. Logistic regression andmultivariate analysis, excluding the impact of gender, age and nationality of the results foundthat these results remained significant (P <0.05, OR=1.985,95%CI of1.388-2.840),The rs1800450locus in the recessive model homozygous and susceptibility to pulmonarytuberculosis related.Conclusions:The SNP on MBL gene, the rs1800450loci polymorphism represents thesusceptibility to PTB among Ningxia populations, which particularly demonstrates increasingthe risk to PTB infection in the recessive model with AA genotypes.