Experimental Study Of High-pressure State EBHM Protection Of Rat Retinal Ganglion Cells

Purpose:1.Establish mixing in vitro culture model of retinal ganglion cells (RGCs) 2.Observe the effect of pressure on the retinal ganglion cell apoptosis 3.Observe the effect of pressure on the and neurotransmitters fleabane investigate the protective effect against high-pressure state retinal ganglion cells possible mechanism.Methods:1. To establish the rat retinal ganglion cells in vitro model of the mixed culture.2.The mixed culture of 24 hours of retinal ganglion cells were grouped with different concentrations fleabane extract and nerve growth factor, according to the experimental design of the test group and the model group sealed and pressurized to 80mmHg, for 4 hours, and cultured for 24 hours measured by enzyme-linked immunosorbent assay (Elisa method) broth nitric oxide, nitric oxide synthase and the content of acetylcholine.Results:1.Mixed culture of retinal ganglion cells can survive for up to 2-3 weeks.2.The ganglion cells pressurized to 80mmHg for 4 hours, and continued to train after 24h, fleabane group nitric oxide concentration was significantly lower than the high pressure model group (P<0.01), inducible nitric oxide synthase was significantly lower than hypertension model group (P<0.01), neuronal nitric oxide synthase in concentrations higher than the hypertension model group (P<0.05), higher than the concentration of acetylcholine hypertension model group (P<0.05).Conclusions:1.Effects of stress can promote apoptosis of retinal ganglion cells and fleabane can fight stress.2.Fleabane extract of retinal ganglion cells under high pressure has a protective effect, the mechanism may be inhibiting the expression of inducible nitric oxide synthase, protect normal expression of neuronal nitric oxide synthase and acetylcholine, reducing nitrous oxide large accumulation of nitrogen to achieve.