| Objective : 1.Establish a fingerprint of Abutilon indicum(L.)Sweet ethyl acetate extract,provide the basis for its spectrum of activity relationship;2.Research on diuretic effect of Abutilon indicum(L.)Sweet ethyl acetate ethyl acetate extract,and to observe the intervention effect of Abutilon indicum(L.)Sweet ethyl acetate extract of liver cirrhosis in rats by intragastric administration;3.By studying the Abutilon indicum(L.)Sweet ethyl acetate extract into blood,to determine the active ingredient of diuretic effect.Methods: 1.Study on fingerprints: Use the method of High performance liquid;HPLC column: Agilent TC-C18(5 μm,4.6 mm × 250 mm);mobile phase: methanol-0.1% phosphoric acid;flow rate: 0.8m L/min;detection wavelength: 226 nm;column temperature: 25 ℃.2.Effect of diuretic: Selection of SPF SD rats were randomly divided into normal control group,hydrochlorothiazide group,Abutilon indicum(L.)Sweet ethyl acetate extract high,middle and low dose group.after intragastric administration for 5 days,at the last administration receiving 24 h urine with metabolic cages rats,urine volume was measured;Research on the intervention effect of Abutilon indicum(L.)Sweet ethyl acetate extract of liver cirrhosis in rats: use SPF SD 100 male rats,after one week of accommodation,10 were randomly selected as the blank group,in addition to the blank group,35% of the remaining animals were given phenobarbital solution as drinking water,one week after the return to normal drinking water,and give 40%CC14-vegetable oil subcutaneous injection modeling.Starting from the second week,subcutaneous injection 40% CC14-vegetable oil solution three times of a week,each time at a dose of 0.2m L/100 g,the first dose is doubled,starting from the tenth around every weekend from modeling animals were sacrificed two rats,as do the liver tissue HE staining,there was observed until cirrhosis,randomly crawl two pumping ascites.If ascites extraction,that determine liver cirrhosis model successfully copied.In after the last administration,ascites was measured,to determine the blood AST,ALT,ALB liver function,serum K + concentration,plasma levels of Ald.3.Selection of SPF SD male rats,high doses of orally administered 120mg/kg,1d/times,for five days,receiving 24 h urine on the fourth day after oral administration,on the fifth day 60 min after intragastric administration to blood collection by the abdominal aortic.Application of HPLC and LC-MS analysis technology to build the method of after oral disc of Abutilon indicum(L.)Sweet ethyl acetate extract containing rat serum samples drugs,by comparing the blank serum,serum containing drug ingredients similarities and differences as well as blank urine,the urine-containing drug liquid ingredients similarities and differences,to determine oral rat Abutilon indicum(L.)Sweet ethyl acetate extract after into blood.Result: 1.Establishment a same pattern fingerprint of Abutilon indicum(L.)Sweet ethyl acetate,and calibrated a total of 10 peaks,10 batches of samples and the control fingerprint similarity are above 0.85.2.The experimental of rats metabolic cages results show that Abutilon indicum(L.)Sweet ethyl acetate extract has a diuretic effect.3.Liver cirrhosis and ascites visually see the model group rat liver wasswollen,uneven surface,showing the size of the nodules,liver cirrhosis histologically false leaflets.4.Compared with the control group,model group significantly increased the amount of ascites(P<0.01)compared with model group,high grass disc ethyl acetate extract to reduce the dose volume of ascites(P<0.01).5.Compared with the control group,he levels of ALT and AST were significantly higher(P<0.01);compared with the model group,Abutilon indicum(L.)Sweet ethyl acetate extract high doses of ALT and AST were decreased(P<0.05).6.Compared with the control group,the positive serum K+ content decreased(P<0.05);no significant difference between each Abutilon indicum(L.)Sweet administered serum K + content than the control group(P> 0.05).7.Compared with the blank group,high dose group,positive group plasma Ald reduced(P<0.05);the other group and the blank group showed no significant difference(P> 0.05).8.Ten of origin of migration into the blood serum component difference is small,its preliminary determination into the blood components from Abutilon indicum(L.)Sweet ethyl acetate extract or its secondary metabolites.Conclusion: 1.the method of fingerprint is simple,accurate and reproducible,it also can be a follow-up study of the relationship between spectral efficiency provide a basis.2.Abutilon indicum(L.)Sweet ethyl acetate extract has a diuretic effect.3.Abutilon indicum(L.)Sweet ethyl acetate extract ascites amount of each dose group was significantly reduced in rats,the drug has a role in eliminating ascites.4.Abutilon indicum(L.)Sweet ethyl acetate extract can reduce levels of ALT and ALP,liver function improved to a certain extent.5.Abutilon indicum(L.)Sweet ethyl acetate extract on rat liver cirrhosis electrolyte imbalance has good corrective action. |
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