The Dynamic Change Of NF-κB And CAMP In The Plasma Of Rats Which Were Received High-voltage Electrical Burn And The Intervention Effect Of Ulinastatin

Objective:High voltage electrical burn has especial and complex pathological characteristics,because of its special causes of injury,and cause loss to body widely.It has complicated complications and so on.Therefore,in the long term clinical work,the treatment of patients with high voltage electrical burns has become an urgent problem to be tackled.Normally,once the body is exposed to high voltage,the current will pass through blood and nerves which have smaller resistance,So the blood vessels and nerve injury is more significant.In terms of peripheral blood vessels,endothelial cell damaged,collagen exposure and massive release of vasoactive substances lead to increased vascular permeability,platelet adhesion and aggregation,all kinds of inflammatory cells increased and micro thrombus formation,etc.Eventually lead to the blood inflammatory state and high coagulation state,influence remote organ tissue microcirculation.CAMP exists in human cells,as the "second messenger" 。cAMP is role in the life of the human body,By participating in cellular metabolic processes.NF-kappa B is involved in the regulation of interleukin,chemokines,adhesion molecules,colony stimulating factor,and other inflammatory small molecules.NF-kappaB signal transduction process involved in the body defense mechanism.This experiment aims to explore the rat,with high voltage electric burn,blood cell nuclear factor kappa B(NF-κB)and cyclic adenosine monophosphate(cAMP)changes,and explore Ulinastatin(UTI)intervention effect on it.Method:1 Animal groups:180 healthy adult male SD rats(Hebei Medical university Experimental Animal Center of certificate No.1411023)were divided into three groups according to the random number table,fake high-voltage electrical burns group(control group),high-voltage electrical burns group(electrical injury group),high-voltage electrical burns UTI treatment group(treatment group).Each group was composed of 60 SD rats.According to the random number table,each group was divided into six phases,15 min before electric shock(T0),5min after electric shock(T1),1h(T2),2h(T3),4h(T4),8h(T5)after electric shock.Each phase was composed of 10 SD rats.Rats were numbered.The rats were fed in different cages.2 Preparation:The rats were weighed,and record data.Experimental drugs were prepared in accordance with the desired concentration.3 Modeling of high-voltage electrical burns: Connect the high-voltage electricity circuit for experimental platform.Intraperitoneal injection of hydrazine hydrate(0.3ml/100g)in rats to anesthetize rats.The rat was fixed on the electric shock test bench.The left and right limbs of rats were connected with 1cm * 1cm electrode,then Left upper limb for entrance,right lower extremity for exit.Turn on electricity,the voltage was adjusted to 2kV,closed switch,disconnect switch after 3 second.According to the experimental requirements,rats in control group were connected with electrode only,didn’t be shocked.Electric injured rats in treatment group were injected of Ulinastatin(20000 units/kg).Rats in electrical injury group and control group were injected of normal saline(5ml/kg).4 Specimen collection and preservation:Rats should to be anesthetized(3ml/kg),then been fixed on an animal operating table.we opened the rat’s chest,drawed out 6ml blood of rats from its heart and injected into the coagulation tube.Static blood 30 minutes,centrifugal blood for 10 minutes with 3000 turn/min.Extracted the upper serum,injected it in Eppendorf tube and stored it in the-70℃ refrigerator.5 Index test:This experiment uses ELISA double anti sandwich method.Detection of cAMP and NF-kappa B concentration in rat serum of different groups.Substrate concentration was calculated from OD value.6 Analysis of Statistics:The data obtained from this experiment were analyzed applying SPSS13.0 statistical software.The experiment data were expressed with the means 士 SD.we used two factors factorial design and multiple comparisons with LSD of t test method.P<0.05 was selected as significant standard.Result: 1 CyclicAMP 1.1 The total content of cyclic adenosine phosphate(cAMP)in the electrical injury group is higher than that in the control group(F=264.71,P<0.05).The content of cAMP is significantly different(F=17.73,P<0.05)in the rats with different phase after injury,and the concentration change with time shows a trend of increasing.The content of 5min-8h after injury was higher than that before injury(P<0.05).1.2 Ulinastatin treatment group of cyclic adenosine monophosphate(cAMP)content is lower than the corresponding phase of electrical injury group(F=23.93,P<0.05).The content of cAMP is significantly different(F=34.71,P<0.05)in the rats with different phase after injury,and the concentration change with time shows a trend of increasing.The content of 5min-8h after injury was higher than that before injury(P<0.05).2 NF-κB 2.1 The total content of NF-nuclear factor kappa B in the serum of electrical injury group is higher than that in the control group(F=1036.16,P<0.05).The content of NF-kappa B in rat with different phase after injury is significantly different(F = 64.60,P<0.05).The concentration change with time shows a trend of increasing.The NF-κB content of 5min-8h after injury was higher than that before injury(P<0.05).2.2 Ulinastatin treatment group of NF-κB content is lower than the corresponding phase of electrical injury group(F=123.54,P<0.05).The content of NF-kappaB in rat with different phase after injury is significantly different(F=128.89,P<0.05).The concentration change with time shows a trend of increasing.The NF-κB content of 5min-8h after injury was higher than that before injury(P<0.05).Conclusion:1 The serum levels of cAMP and NF-nuclear factor kappa B are increased in different degrees after high-voltage electric burn,proved that the high pressure burn can heighten the inflammatory state of the blood and promote various types of blood cell aggregation,adhesion,adhesion,etc,so that the body is in a state of high coagulation.2 The serum levels of cAMP and NF-nuclear factor kappa B in treatment group are lower than that of high voltage electrical injury group.Tip UTI can help reduce the level of NF-nuclear factor kappa B and cAMP after electrical burn.Reduce the body’s inflammatory reaction and high blood coagulation state,prevent thrombosis.