Effect On The Content Of β-endophine In The CCI Model Muscle That Manipulation Operating On

Objects:To observe the influence of pain threshold in CCI model and the content of β-endophine in the CCI model muscle that manipulation operating on.Try to explain the analgesic mechanism of manipulation in the treatment of neuropathic pain,looking for the theoretical basis for manipulation analgesia.Methods:32 healthy SD female rats were randomly divided into four groups: Sham-operated group,CCI group,Acupuncture group and Manipulation group.Four groups,eight rats in each group.(1)Sham-operated group:Making model by referring to CCI model making method without Ligation of sciatic nerve。Subsequently,Stripping sciatic nerve,washing and suture.Don’t carry out any treatment on the Sham-operated group.The heat pain threshold was measured at third day and sixth day respectively.At the sixth day after the operation,the rats were put to death and Taken specimen.(2)CCI group:Making model by referring to CCI model making method,Don’t carry out any treatment on the CCI group.The heat pain threshold was measured at third day and sixth day respectively.At the sixth day after the operation,the rats were put to death and Taken specimen.(3)Acupuncture group:Making model by referring to CCI model making method.The heat pain threshold was measured at third day and sixth day respectively.Begining acupuncture treatment at Sixth day after operation.In the quiet environment,the rats were fixed on the special animal clamp.The head and body are fixed,and the animal is selected to acupuncture the Huan Tiao acupoint in the waking state.The depth of acupuncture is 0.5cm.hand-manipulating of needle once time Every 5mins,a total of 20 minutes.After treatment,it was put to death and taken specimen.(4)Manipulation group:Making model by referring to CCI model making method.The heat pain threshold was measured at third day and sixth day respectively.The head and body are fixed,and the animal is selected to chirapsia the Huan Tiao acupoint in the waking state.The performer to use the index finger chirapsia the Huan Tiao acupoint,a total of 20 minutes.After treatment,it was put to death and taken specimen.By measuring the rat limb salvage operation of thermal shrinkage foot reflection and observation of rats with limb thermal pain threshold and the general situation,assess whether the CCI model was successful.1 days before operation,3 days after operation,6 days after operation,after manipulation treatment do heat pain threshold acquisition.Detecting the content of beta-endorphin with ELISA kit after manipulation treatment.Analysis of the influence of content of beta endorphin in the rates muscle.Analysis manipulation how impact content of β-EPResults:1.The general physical condition of rats.The rats of the sham-operation group fur was smooth,good mental state,dynamic Hi,sensitive reaction,normal diet;The rats of CCI group,Acupuncture group and Manipulation group became apathetic,less activity,poor diet.Operation side became limping,lifting the foot,and licking foot.2.Comparison of body weight of rats in each group were made before operation,there was no statistically significant difference(P > 0.05),After modeling,increase body weight of CCI rats is slow,there was significant difference compared with the sham operation group(P < 0.05)..3.Before making model rats between the thermal pain threshold comparison,no significant difference(P > 0.05);3 days after operation,Compared with the sham operation group,CCI group,Acupuncture group and Manipulation group,three groups of pain threshold were significantly decreased,there were significant differences(P < 0.05);Compared with pretherapy,post-treatment elevate pain threshold,there was significant difference(P < 0.05).Compared with CCI group,Acupuncture group and Manipulation group there was significant difference(P < 0.01).Compared with acupuncture group,Manipulation group was’ t significant difference(P>0.05)contrast of theβ-EP concentration After manipulation treatment : The concentration of beta-EP was significantly higher than pretherapy;Compared with the sham-operation group,CCI group was’ t significant difference(P>0.05),Acupuncture group and Manipulation group was significant difference(P < 0.01)Compared with CCI group,Acupuncture group and Manipulation group were significant difference(P < 0.05).Compared with acupuncture group,Manipulation group was’ t significant difference(P>0.05).Conclusion:1.Manipulation has analgesic effect what can reinforce pain threshold of CCI model rats.2.Manipulation can increase the content of β-EP in muscle of CCI model where manipulate at.3 The analgesic mechanism of manipulation is by increasing the content of β-EP to get analgesic effect.probably.