A Discussion On The Expression Of BS69 In Colorectal Cancer And Its Clinical And Pathological Correlation

Objective: To detect the expression level of BS69 in human colorectal cancer and explore the relationship between the expression of BS69 and the clinical and pathological characteristics of colorectal cancer patients.Methods: Immunohistochemical assay was used to detect the expression of BS69 in 50 specimens of colorectal cancer tissues and 30 specimens of adjacent normal colorectal tissues, while western blot was used to detect the expression of BS69 in four human colorectal cancer cell lines(SW480, HT29, HCT116, DLD1) and one normal colorectal epithelial cell line FHC.Results: There were significant differences in positive expression rates of BS69 between colorectal cancer tissues and adjacent normal colorectal tissues(70.0% vs 93.3%, p<0.05). In almost every patient, the expression level of BS69 in colorectal cancer tissues is higher than its adjacent normal colorectal tissues, whatever the histological grade is. Besides, the expression of BS69 in colorectal cancer tissues is negatively correlated with histological grade, Dukes stage, lymphnode metastasis and distant metastasis(p<0.05), but not correlated with age and gender of patients, tumor size and tumor locations(p>0.05). The obvious differences in the expression level of BS69 were also observed between four human colorectal cancer cell lines with western blot assay. Of the four colorectal cancer cell lines, SW480 has the highest BS69 protein contents, which is much higher than normal colorectal epithelial cell line FHC. While HCT116 and DLD1 have the lowest BS69 protein contents, much lower than FHC.Conclusion: Decreased expression of BS69 may be involved in the development, invasion and metastasis of colorectal cancer. Detecting the expression of BS69 is valuable for estimating the clinical development and prognosis.