| Objective:To evaluate the biocompatibility of MC3T3-E1 on the surface of titanium alloy treated by micro arc oxidation alkali treatment and chitosan alginate coating.Study different disinfection methods of material surface activity,for pure titanium surface by microarc oxidation alkali treatment,chitosan sodium alginate composite coating,high temperature and high pressure ultraviolet irradiation disinfection methods were used to detected the effects of the growth of osteoblast MC3T3-E1.Methods: 1 The titanium plate is treated by alkali-micro-arc oxidation,then separately of chitosan and the sodium alginate coating processing.Pure titanium micro-arc oxidation group as control group A,pure titanium micro-arc oxidation-alkali treatment-chitosan composite treatment for experimental group B,pure titanium micro-arc oxidation-alkali treatment-chitosan and sodium alginate composite processing for group C.CCK 8 experimental tests cell adhesion and proliferation at different times Laser observes confocal cytoskeleton form early detection,scanning electron microscopy(sem)observation specimens surface morphology,ALP detection of the different time of alkaline phosphatase activity.2Acording to the result of experiment 1,group C is the best to improve the growth of the cell,so we choose the group C to continue the next experiment : pure titanium micro-arc oxidation-alkali treatment-chitosan and sodium alginate composite processing of the composite coating on osteoblast adhesion proliferation and mineralization have better promoting effect,on the basis of the experimental group C composite coating specimen respectively high temperature and high pressure disinfection(high temperature and high pressure),hereinafter referred to as HTP group and ultraviolet disinfection(ultraviolet)U group,observation of different disinfection methods for composite coating on the surface of osteoblast adhesion proliferation and mineralization.Results: Experiment 1 adhesion experiments show that the adhesion of two groups of B,C on the absorbance value difference was not significant,were superior to group A;Proliferation experiment group C of the surface of the specimen proliferation rate is significantly higher thanB,A,in the two groups.By observation of the cytoskeleton,at the same point in time,group C than group B cells showed a better ability to survive,pseudopodia,pseudopodia length,are better than that of group B cell spreading area,shows the better guide cell interaction trend;Alkaline phosphatase activity was significantly higher than B,A group C in the two groups.In the adhesion experiments in experiment 2 U adhesion value superior to HTP group,the same time on the proliferation rate of U group of proliferation degree is obviously better than that of HTP group.Cytoskeleton morphology observation,two groups of specimens of the same time,U cell spreading area,number of pseudopodia,pseudopodia number also is better than that of HTP group,were observed under scanning electron microscopy(sem)HTP group specimen surface has obvious cracks and defects,and U set of specimen surface integrity,and not after disinfection of the original specimen surface have no obvious difference.Conclusion:1Pure titanium micro-arc oxidation-alkali treatment-chitosan and sodium alginate composite coating for cell have a significant role in promoting adhesion,proliferation and alkaline phosphatase expression,shows that chitosan-sodium alginate coating is helpful to the early stages of the implanted implant stability as well as the early mineralization,is an ideal implant coating material.2 Pure titanium micro-arc oxidation-chitosan/alginate sodium biological coating adopts high temperature and high pressure disinfection method,the surface damage of the material,make the material surface structure change,loss of surface film layer effect,and surface properties of ultraviolet disinfection way without breaking.Adopts ultraviolet disinfection method,coating surface bioactivity than high temperature and high pressure steam sterilization disinfection way,conducive to cell adhesion and growth,suggest material surface for biological macromolecule material,use ultraviolet disinfection method. |
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