| Objective:To observe the effect of Modified Decoction of jiajiansanjiasan on hepatic angiogenesis in rats with immunological liver fibrosis, and to explore the effect and mechanism of jiajiansanjiasan on anti hepatic fibrosis.Methods:24 Wistar rats were randomly divided into control groups,model groups,positive control groups and Jiajiansanjiasan groups. After 7 days common breeding,all the rats were duplicated immuno-hepatic fibrosis model by injecting porcine serum intraperitoneally except the control groups. After modeling, all the rats were taken medicine by gavage, once daily.The medicine of Jiajiansanjiasan groups were taken jiajiansanjiasan powder, positive control groups were taken fufangbiejiaruanganpian powder,control groups and model groups were taken with sovolumetric 0.9%NaCl. After 60 days remedy, taking rats liver, dyeing by HE and MASSON, staining were liver pathological morphology, CD34 markers detection of hepatic microvascular density, Western Blot method in detection of liver tissue VEGFA/VEGFR-2 protein, RT-PCR method to detect liver tissue CD44, vWF, VEGFA/VEGFR-2 mRNA expression level. Using SPSS 19.0 statistics software to analysis on the experimental data, the comparison between groups, when P< 0.05, the difference was statistically significant.Results:1. Hepatic pathology observation of the model ratsLiver pathology observation:after HE and MASSON staining, liver tissue pathological model group then has central vein and portal area fibrous tissue hyperplasia obviously, compared with normal group have obvious fiber bridge connection and false flocculus to form. Two fiber hyperplasia drug therapy group was obviously relieve, portal area only a small number of relatively thin collagen fiber split hepatic lobule.2. Microvessel density (MVD) MVD of rats model group was obviously higher than that of normal group (P< 0.05), the jiajiansanjiasan group's MVD decreased significantly than the model group (P< 0.05).3.The VEGFA/VEGFR-2 signal pathwayModel group VEGFA protein and mRNA expression level significantly higher than normal group (P< 0.05), the expression of VEGFA/VEGFR-2 protein and mRNA of jiajiansanjiasan group were reduced (P<0.05), treatment group VEGFA/VEGFR-2 protein and mRNA expression have no difference (P> 0.05).4.Adhesion molecules CD44 and vWFThere was no significant difference between the CD44 model group and the normal group (P>0.05), and there was no significant difference between the model group and the jiajiansanjiasan group (P>0.05). Compared with the normal group, model group vWF expression obviously increased in comparison with the model group (P< 0.05), and jiajiansanjiasan group and Biejia tablet group vWF expression was significantly decreased (P< 0.05).Conclusion:1.Three MVD scattered groups of rats were significantly lower than that of model group rats MVD, suggest to add and subtract 3 armour inhibits pathologic hyperplasia of capillaries, may be one of the mechanisms of anti liver fibrosis, suggest to add and subtract 3 armour inhibits pathologic hyperplasia of capillaries, may be one of the mechanisms of anti liver fibrosis.2.The expression of VEGFR-2 protein and mRNA were lower in the model group than in the model group, which suggested that the modified decoction could inhibit the liver pathological angiogenesis in rats with hepatic fibrosis,it is possible to improve the expression of VEGFR-2 protein and mRNA by improving the microcirculation of the liver in rats, and further influence the related signal pathway VEGFA/VEGFR-2 of pathological angiogenesis.3.The expression of CD44 in liver tissue was not obvious, and no specific. The expression of vWF in the sanjiasan group was significantly decreased, suggesting that the addition and subtraction of jiajiansanjiasan can inhibit the expression of vWF in hepatic vascular endothelial cells, and the mechanism of anti fibrosis may inhibit the expression of vWF in hepatic vascular endothelial cells. |
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