Significance Of Levels Of MMP-13 Detecting In Plasma Of Patients With Multiple Myeloma

Multiple myeloma(MM)is a kind of plasma cell monoclonal malignant proliferative disease,it can cause extensive bone destruction,anemia,recurrent infection,renal insufficiency and a series of other clinical manifestations.Myeloma bone disease(MBD)is one of the characteristic lesions of MM.It is mainly manifested as bone pain,hypercalcemia,osteoporosis,osteolytic destruction and pathological fracture,which seriously affects the life quality of patients and disease prognosis.The main pathogenesis of MBD is believed to be a combination of osteoclast overexpression and osteoblast inhibition,leading to bone resorption and bone formation imbalance.Matrix metalloproteinases(MMPs)are a family of zinc-dependent endopeptidases with proteolytic enzyme activity.In recent years,the study found that MMP was an important factor in the development of MBD.MMP-13 is an important member of the MMP family and is associated with some processes such as bone reabsorption,tumor infiltration and metastasis.However there is little relevant research about the relationship between MMP-13 and MBD.This study detected the plasma levels of MMP-13 in 53 newly diagnosed MM patients by enzyme-linked immunosorbent assay(ELISA),and analyzed the relationship between MMP-13 and severity of bone disease,bone mineral density,ISS stage.The purpose of this study is to explore the potential role of MMP-13 in the development of MBD,MM condition judgment and prognosis evaluation.Objective:To investigate the significance of detecting the plasma levels of MMP-13 in MM patients and preliminarily explore the potential role of MMP-13 in the development of MBD,MM condition judgment and prognosis evaluation.Methods:The plasma levels of MMP-13 were quantified in 53 newly diagnosed MM patients and 30 healthy controls by ELISA.Imaging examination was used to determine bone damage in these patients with MM.At the same time,using dual-energy X-ray absortionmetry(DXA),the bone mineral density was examined on vertebrate L2 to L4 in the anteroposterior position and the proximal left femur in 17 MM patients and 15 healthy controls.And keeping detailed records on ISS stage andβ2-microglobulin(β2 MG),albumin,hemoglobin,serum creatinine and other clinical indicators in 53 newly diagnosed MM patients.Results:(1)The plasma levels of MMP-13 in MM patients were significantly higher than that in the controls(P<0.01).(2)The levels of MMP-13 in MM patients without MBD were significantly higher than that in the controls(P<0.05).(3)According to bone disease grading,the 53 patients were divided into group A(bone grade 0-2,n=18)and group B(bone grade 3-4,n=35).Compared with group A,group B had higher MMP-13 levels(P<0.01).(4)Further analyses revealed that the levels of MMP-13 had negatively correlation with the bone mineral density on L2 to L4,greater trochanter and Ward’s triangle(γvalues were-0.693,-0.575 and-0.575,respectively,P<0.05),but no association with left femoral neck(γvalue was-0.339,P>0.05).(5)The levels of MMP-13 in MM patients with stage Ⅲ of International Staging System(ISS)were significantly higher than in patients with stageⅠ-Ⅱ(P<0.01).(6)MMP-13 levels were positively correlated with β2-MG,serum creatinine(γvalues were 0.451,0.352,P<0.05),and had a negative correlation with hemoglobin levels(γvalue was-0.317,P<0.05).MMP-13 levels had no correlation with albumin,LDH(γvalues were-0.269,0.242,P>0.05).Conclusion:The levels of MMP-13 in MM patients are significantly elevated,and closely related to the degree of MBD,the bone mineral density of MM patients and ISS clinical stage.Our study reveals MMP-13 play an important role in the development of MBD,MM condition judgment and prognosis evaluation.