| Background and purpose:Diabetic nephropathy is the most common complication of clirical diabetes mellitus.It has the characteristics of rapid progress and difficult treatment,and the prevalence rate is gradually increasing in recent years.Proteinuria is the main clinical manifestation of diabetic nephropathy,from early microalbuminuria to a large number of proteinuria in the late,has been confirmed that proteinuria is an independent risk factor for the progression of diabetic nephropathy,so to explore the pathogenesis of proteinuria and find a lower proteinuria The method of protecting renal function is particularly urgent.Previous studies on the pathology of diabetic nephropathy mainly focus on glomerular hypertrophy,basement membrane thickening,mesangial cell proliferation and matrix enlargement,sclerosis and so on,but these pathological changes can only explain some of the clinical manifestations,more The cause is still unclear,in recent years,people will study the direction of the glomerular podocyte above,because the podocyte is the last layer of renal filtration barrier,not only with the mechanical barrier related to the charge barrier,so sometimes become Hot spot.Podocytes play a vital role in maintaining glomerular filtration and preventing leakage of proteinuria.Any form of injury,whether it is autoimmune response or drug toxicity can lead to podocyte damage,thereby affecting its function.Podocyte injury mainly manifested as cell hypertrophy,foot process disappeared,the number decreased or even shedding apoptosis.Adjacent to the foot between a similar zipper-like structure of a layer of the diaphragm,the diaphragm is composed of a variety of molecules combined with each other,the hole is the last layer of membrane filtration,if its integrity is destroyed Will lead to leakage of proteinuria,hyperglycemia in vivo,AGEs.oxidative stress can cause podocyte damage to the hole diaphragm damage.Experiments show that the number of early podocytes in the model of glycogenin is reduced,the molecules on the diaphragm Nephrin,Podocin protein content will decline,to the late this change is more obvious.Podocyte injury and glomerular sclerosis also have a relationship.Donblier et al.observed in the pathogenesis of glycogen in early Nephrin expression can be abnormal,and with the occurrence of proteinuria and glomerular filtration have a significant correlation,so the study of podocyte injury and diabetic nephropathy,For the clinical prevention and treatment of diabetic nephropathy to provide a new basis.My teacher Professor Yao Yuanzhang engaged in clinical diagnosis and treatment work for nearly four decades,good at the prevention and treatment of kidney disease,especially for diabetic nephropathy has its unique insights.In combination with years of clinical experience and the incidence of diabetic nephropathy based on the characteristics of the creation of a syrupy kidney mixture,the party to cut the pathogens,taking into account the actual situation of the specimen,the main Yin and yin deficiency,the standard to blood stasis,phlegm,Poison so that,so the side has an appetite Sheng Jin,Tongluohuoxue Gong,in clinical clinically achieved satisfactory results.In the previous experiment,we confirmed that the side has a regulation of JAK/STAT pathway,regulation of renal tissue SOCS-1,TGF-β1 and VEGF expression to protect the role of the kidneys.Does the action mechanism of reducing the proteinuria are related to the regulation of the expression of nephrin and podocin in podocytes?The experimental group was divided into model group,treatment group and normal control group by animal model.Finally,the protective effect of Tangxian mixture on kidney of diabetic rats was analyzed by measuring the contents of nephrin and podocin in each group.And its possible mechanism.The research methods:1.Establishment of diabetic rat model:SD rats were given high-fat and high-fat diet 5 weeks after intraperitoneal injection of low-dose streptozotocin(35mg/kg);model standard:rats without fasting blood glucose>16.7mmol/L The2.Grouping:The rats were divided into normal group,divided into model group,control group and Tangxian mixture high and low group of 5 groups.3.Dosage method:according to the dose of 15ml/Kg/d in the form of gavage were given each group of the corresponding liquid.Dosing for 8 weeks.4.Evaluation of the efficacy:After 8 cycles of administration,the rats were sacrificed and the rats were sacrificed at the end of the rats.Blood samples were collected and the rats were sacrificed.Blood samples were collected and measured for 24 hours.(Serum creatinine,serum albumin and urea nitrogen)were taken from the abdominal aorta and the pathological changes of renal tissue were observed by HE staining.Western blotting and polymerization The expression of nephrin and podocin protein and the content of nephrinmRNA and podocin mRNA in renal tissue of diabetic rats were detected by enzyme-linked chain reaction(PCR).Semi-quantitative analysis was performed by image analysis software(Image-Pro Plus).SPSS 17.0 was used to analyze and analyze the data,P<0.05 was statistically significant,P<0.01 was statistically significant.Results:1.The amount of protein in the model group was significantly higher than that in the normal group,and the protein level of the control group was lower than that of the model group.2.The levels of creatinine and urea nitrogen in the model group were significantly higher than those in the normal group,and the levels of creatinine and urea nitrogen in the syrup group were lower than those in the model group.3.Serum albumin:serum protein was significantly lower in the model group than in the normal group,and the serum protein of the control group was higher than that of the model group.4.The content of nephrin and podocin in the renal tissue of the model group was significantly lower than that of the normal group(p<0.01).The expression of nephrin and podocin in renal tissue was higher than that in the control group(P<0.01).Compared with the normal group,the expression of nephrin and podocin was decreased(p<0.01).5.Pathological changes:light microscopy can be seen in the normal group of glomerular morphology is clearly visible,no significant cell proliferation,mesangial area was no significant widening,capillaries without compression,no balloon adhesions,tubule stroma no fibrosis The In the model group,glomerular hypertrophy was observed under light microscope,the mesangial area was widened,the basement membrane was thickened,some balloon adhesions and fibrosis were observed.The changes of glaucoma group and losartan group were less than those in the model group,and the pathological changes of kidney were the most obvious in the group of Sugar and Kidney and losartan.Conclusion:Tangxian mixture can reduce the amount of urine protein,increase serum protein content,reduce the degree of creatinine nitrogen in different degrees and reduce the pathological changes of renal tissue.The protective mechanism of Tangxian mixture on kidney may be related to the up-regulation of nephrin,podocin mRNA and protein expression in renal tissue. |
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