Systematic Reviews And Differential Proteomics Analysis On Liver Fibrosis Induced By Arsenic

Objective: Using the method of bibliometrics and statistical principle,the2005~2016 published at home and abroad on the theme "arsenic pathogenic mechanism" in the literature of quantitative analysis and qualitative analysis,research status,focus and development trends at home and abroad in order to reveal the pathogenic mechanism of arsenic;Using the method of meta analysis of the relationship between hepatic fibrosis and exposure assessment of arsenic based on epidemiological survey data;Using 8standard isobaric tags for relative and absolute quantitation(isobaric tags for relative and absolute quantification,iTRAQ)technique combined with two-dimensional liquid chromatography tandem mass spectrometry(2DLc-MS/MS)technique to filter out the difference induced by arsenic in rats with liver fibrosis of the protein,and carries on the analysis bioinformatics,providing data support for the elucidation of the mechanism of liver fibrosis induced by arsenic.Methods: Using the literature management software ENDNOTE published in 2005 ~ 2016 at home and abroad on the subject of "arsenic pathogenesis" of the literature published year and source journals analysis,Using the information visualization software CiteSpace on the Web of ScienceTM core collection and CNKI core Journal of the journal in the same period of time in the field of literature were cited,co-occurrence,co-word,cluster analysis.Second,the computer retrieval CNKI full-text database,Wanfang full-text database,Chinese biomedical literature database,Vipu database,PubMed database,web of science library,EMBASE database,search terms for arsenic exposure and liver fibrosis,retrieval time from the building to 2016 On December 31,the literature was selected according to the inclusion and exclusion criteria,the data were extracted and the quality of the included literature was evaluated.The mata analysis was performed using Stata12.0 software to calculate the combined OR and 95%CI values,and the sensitivity analysis and publication bias Evaluation.The rat model of liver fibrosis induced by sodium arsenite was established.The differential proteins were screened by using 8-labeled iTRAQ technique and 2DLc-MS/MS.The differential proteins were inserted into Uniprot and STRING 10.0 to identify the genes name,function and involved Pathways,Useing Goanna software to analyze the biological processes involved in the differential proteins,the cell location and molecular functions.On the basis of the GO annotation analysis,the biological processes involved in the differential proteins were clustered by R software to screen out the main biological processes.Results:1.The development of arsenic pathogenesis research at home and abroad is relatively balanced,with slight fluctuation during the period,and the average number of published papers is 227-260.However,the number of published papers in this field has been declining since 2013.2.The journals published in domestic and foreign literature are more than 10 journals are core journals,93% of foreign journals JCR distribution in Q1,Q2,37% of the journal of the impact factor is greater than 5,the research areas are mainly concentrated in pharmacology,toxicology,biochemistry And molecular biology and other fields.3.With the H index and high cited results analysis,arsenic pathogenesis in the field of academic achievements in the top four authors for the Styblo Miroslav,Vahter Marie,Thomas Daniel J,Aposhian HV;play a key role in the top three authors For Yamanaka K,Anonymous and Mass MJ;both classical and critical papers are A new metabolic pathway of arsenite: arsenic-glutathione complexes are substrates for human arsenic methyltransferase Cyt19 and Trivalent arsenicals are bound to proteins during reductive methylation.The first three countries are the United States,China and Japan,the countries with the closest degree of cooperation are the United States,Canada,the United States,Canada,the United States,Canada,the United States,Canada,the United States,Canada,the United States,Canada,And Germany;institutions in Arizona University research results up.4.The author of the first three co-author of the domestic co-author Zheng Yujian,Wang Jiying and Wu Jun,co-author of the research results of the first institutions of Guiyang Medical College Affiliated Hospital Infectious Diseases.5.Pathogenic mechanism such as arsenic toxicity,oxidative stress,methylation,cell transformation,arsenic trioxide,signal transduction,protein glutathione and mechanism research more commonly recorded in foreign researchs,a research hotspot in the field of the research on the mechanism of arsenic trioxide,apoptosis,sodium arsenite and arsenic poisoning.6.Expression,demethylation,arsenic acid,bladder cancer,arsenic methylation is a frontier research field of research on pathogenic mechanism of arsenic in different time.7.Arsenicpathogenic mechanism of arsenic trioxide,methylation,intake,dose and other 6 items.8.System reviews of arsenic exposure and fibrosis the liver showed that the heterogeneity test results of ? 2=9.27,P=0.234(>0.10),I2=24.5%(<50%).There were significant differences in Z=8.42,P <0.01,arsenic exposure and non arsenic exposure to hepatic fibrosis,the difference was statistically significant.Results the combined OR value was3.50,95%CI was 2.615-4.685,and the incidence of hepatic fibrosis in arsenic exposed area was about 3.5 times that of non arsenic exposure area.9.The study found that Hic-5protein,glutathione synthetase in the middle and high dose group were increased,there are six kinds of tropomyosin protein,the down-regulation of Tmp2 in the high dose group,the other five showed up.The signal transduction pathway closely related to liver fibrosis is MAPK pathway,PPAR gamma-NF kappa B signaling pathway,TGF-beta pathway,Wnt signaling pathway.The main biological processes of differential proteins are metabolism,including amino acid synthesis and metabolism,glucose metabolism,fatty acid synthesis and metabolism.Conclusion: 1.The application of computer text mining technology to extract arsenic in the pathogenesis of the status quo,hot spots and topics,from the perspective of the overall analysis and control,to provide a basis for further research literature.2.Arsenic exposure is associated with the occurrence of hepatic fibrosis,suggesting that arsenic exposure may be a risk factor for the high incidence of liver disease.3.Hic-5 protein,glutathione synthetase,tropomyosin and liver fibrosis is closely related to MAPK pathway,PPAR y-NF B pathway,TGF-pathway,beta Wnt signaling pathway plays an important role in the regulation of hepatic fibrosis.Glucose metabolism amino acid synthesis and metabolism;Fatty acid synthesis and metabolism,are all involved in liver fibrosis induced by arsenic.