Genetic Polymorphism Of MiRNA-146a And Susceptibility To Bladder Transitional Cell Carcinoma

Bladder transitional cell carcinoma(BTCC)is one of the most common malignant tumors of the urinary system.It has high recurrence rate and mortality,which seriously affects human life and health.The occurrence of bladder cancer and genetic susceptibility,environmental factors are closely related.In recent years,with the development of genomics,it has been revealed that the single nucleotide polymorphisms(SNPs)of mi RNA genes are closely related to the development of various diseases.Single nucleotide polymorphisms(SNPs)are the most common gene mutations in the human genome,pre-mi RNAs and / or mi RNAs sequences may lead to changes in mi RNA expression and changes in maturation.Mi RNA-146 a is located in the second exon region of the human chromosome fifth LOC285628 gene,which plays an important role in gene regulation,cell proliferation and immunoregulation.By mi Rbase query,mi R-146 a G> C(rs2910164)in the Chinese population of the variability among the top three,not less than 5%;It was found that the polymorphism of Mi R-146 a G> C(rs2910164)gene wasclosely related to the risk of breast cancer,gastric cancer,lung cancer,and liver cancer.In this study,the relationship between mi R-146 a gene polymorphism and bladder transitional cell carcinoma was investigated by case-control study.Objective: Based on the analysis of polymorphism of Mi R-146 a G> C gene rs2910164 in Han population of western China,to understand the allele and genotype distribution of the gene in the Han population,and the relationship between the polymorphism and transitional cell carcinoma of the bladder.Methods : Blood samples were collected from 212 cases of bladder cancer patients and 291 healthy subjects in western China by case-control study.Genomic DNA was extracted from the study.The genotype of mi RNA-146a(rs2910164)was detected by PCR-RFLP,according to the DNA sequencing to verify the results.Taking statistical analysis by useing SPSS22.0 software.(Rs2910164)loci were analyzed by t-test or x2 test in the control group and the case group.The unconditional logistic regression model(corrected age,sex,smoking and drinking factors)was used to analyze the gene(rs2910164)(OR),95% confidence interval and P value,and further stratified analysis of environmental factors.The unconditional logistic regression model(corrected age,age,age,and age)were used to analyze the risk of cancer genetic susceptibility in different regions of China.Sex,smoking anddrinking factors).The relationship between different gene models and staging,classification and metastasis of bladder cancer was analyzed.The interaction between bladder risk factors and gene-environment factors was analyzed by unconditional logistic regression.Results:1.The distribution of sex and age in the two groups are not statistically different(P> 0.05).The distribution of smoking and drinking is statistically different(P <0.05).The goodness of x2 test showed that the control group(rs2910164)Genotype distribution is consistent with Hardy-Weinberg equilibrium,indicating that the control group of people with group representation.2.rs2910164 polymorphism in the C allele is associated with increased risk of bladder cancer(additive model OR = 2.77,95%CI = 2.07-3.70,P <0.001;The allele model OR = 2.55,95% CI =1.97-3.30,P <0.001);Individuals with GC / CC genotype had an increased risk of bladder cancer(dominant model OR = 3.40,95% CI = 2.15-5.36,P <0.001;Recessive model OR = 4.00,95%CI = 2.50-6.39,P <0.001).3.In the recessive gene by age,sex,smoking and drinking and other factors stratified analysis found that Mi R-146 a CC will increase the risk of bladder cancer.4.There is no correlation between the polymorphic loci and the clinical features(staging,grade and metastasis)of bladdertumor.There is no significant difference between the two groups,P values were greater than 0.05.5.Smoking and drinking are the risk factors of bladder cancer,P value is 0.000,0.032,environmental factors smoking,drinking and genotype GC,CC in the occurrence of bladder cancer has a certain interaction(?=0.662,P=0.003;?=1.737,P<0.001);(?=0.747,P=0.003;?=1.155,P=0.008).Conclusion: The dominant model,the additive model and the recessive model in the Mi R-146a(rs2910164)GC/CC genotype increase the risk of bladder cancer,the allele model C allele also increases the risk of bladder cancer.Smoking,drinking are the risk factors of bladder cancer,there is a positive interaction between environmental factors,smoking,drinking and genotype GC,CC.