The Biological Effect Of Bone Marrow Mesenchymal Stem Cells Transplantation On Ovariectomized Rats

Objective The SD rats bilateral ovaries to establish postmenopausal osteoporosis model,intravenous injection of bone marrow mesenchymal stem cells to ovariectomized rats via tail vein,to reasech the effects of bone marrow mesenchymal stem cells stem cell transplantation on ovariectomized rats,on bone mineral density interleukin-10(IL-10)、osteoprotegerin(OPG)and bone source alkaline phosphatase(BLAP),to provide more theoretical basis for stem cell transplantation in the treatment of postmenopausal women with osteoporosis.Methods Forty-seven healthy SD rats from 6 weeks of age,provided by experimental animal center of Bengbu Medical College,raised after 2 week,randomly selected 2 rats were used for BMMSCs extraction,the other rats were randomly divided into 2 groups: normal control group(group Sham,n=15)and ovariectomized group(OVX group,n=30).Ovariectomized group after back surgery incision with bilateral ovaries to establish the model of postmenopausal osteoporosis in the normal control group underwent the same surgical exposure and resection of bilateral ovarian volume of adipose tissue,but bilateral ovarian reserved.All rats can double the initial BMD of proximal femur determination by X-ray absorptiometry and the serum levels of IL-10,OPG and BAP were measured by using ELISA kit to detect blood samples from the tail vein to establish a baseline.Six months after surgery,measured all rat’ BMD of proximal femur in vivo by dual energy X-ray absorptiometry and test serum IL-10,OPG and BAP level Again.Then the ovariectomized group were randomly divided into 2 groups(n=15,A group and B group),A group(OVX+A)by intravenous injection of bone marrow mesenchymal stem cells about 3*106,group B(OVX+B)inject phosphate buffer in the same volume(PBS).The normal control group(Sham)treated alike B group(OVX+B).Two months after,measure bone density of all rats and interleukin-10,osteoprotegerin and bone alkaline phosphatase levels.Results 1、The ovariectomized rats proximal femoral bone density was significantly lower than the normal control group,the difference was statistically significant(p<0.05).The ovariectomized group,the bone marrow mesenchymal stem cells in the treatment group bone density has improved compared with the positive control group,but still lower than the normal control group,with statistically significant difference between the groups(p<0.05)。 2、Six months after operation,the level of serum IL-10 to rats ovary decreased compared to normal control group;after transplantation of bone marrow mesenchymal stem cells after treatment,the serum level of IL-10 in a group than in B group increased,the difference was statistically significant(p<0.05).3、 The serum level of OPG in ovariectomized group is lower than normal control group,after treatment of bone marrow mesenchymal stem cells,serum levels of bone protection in a group was higher than that in B group,a statistically significant difference(p<0.05)4 、 The ovariectomized rats serum bone alkaline phosphatase levels increased compared to normal control group,the difference was statistically significant(p<0.05).There was no significant difference in serum level of bone alkaline phosphatase between group A and B group before and after treatment with bone marrow mesenchymal stem cells(p>0.05).Conclusion 1、Six months after surgery,the ovariectomized group compared with normal control group,proximal femoral bone density was significantly lower,verify the ovariectomy to establish postmenopausal osteoporosis disease model is effective and reliable.The improved bone density after treatment showed that bone marrow mesenchymal stem cell transplantation can promote bone formation。 2.After treatment of bone marrow mesenchymal stem cells in ovariectomized rats,serum IL-10 and osteoprotegerin levels tended to normal control group,showed that bone marrow mesenchymal stem cells on the positive regulation of bone metabolism.3、Although bone marrow mesenchymal stem cell transplantation is unable to completely repair,but it is expected to provide a new selection for the treatment of postmenopausal osteoporosis.