To Investigate The Influence Of DXR On Blood Vessel Formation In AS Plaque Via Ang1-Tie2

BackgroundAcute coronary syndrome(ACS)is the main performance of the cardiac events in patients with coronary heart disease,its common pathophysiological basis is coronary artery atheromatous plaque become unstable and fracture under the action of patches in a variety of factors,then blood clots would create.According to new research,neovascularization has the relationship with the atherosclerotic plaques progressed and stability.The Angiopoietinl/Tie2 is the important system which has emerged as an essential event in the maintenance of a quiescent vasculature.VEGF is one of the best known of the main inducing angiogenesis growth factors,VEGF can not only specific effect on vascular endothelial cells,strongly promoting the proliferation,differentiation and induce angiogenesis in vivo,but also can increase microvascular permeability,and promote plasma fibrin extravasation.Furthermore,VEGF may also has the great significance in differentiation of vascular structures,reconstruction and maintenance.Angl/Tie2 system and VEGF have the precision balance,It plays an important role in adjusting blood vessel growth and maintain the integrity of the existing blood vessels.Chinese scholars think,"Qi,blood stasis,phlegm"are the most important characteristic of the AS.The main methods of TCM Prevention and treatment of AS Blood stasis is Qi and Phlegm Blood stasis.Our school affiliated hospital of traditional Chinese medicine is DingXin party,a beneficial qi and activate blood circulation dry wet expectoranting function.Panax notoginseng saponins R1,as one of the important components of notoginseng,which is one of composition TCM of DXR,studies have shown it also can significantly change the pathological process of atherosclerotic plaque.ObiectiveThis study aims to investigate the influence and possible anti—atherosclerosis mechanism of DXR and PNSR1,through the simulation of angiogenesis in vitro,to observe the DXR and PNSR1 angiogenesis inhibition on Blood vessel formation in human umbilical vascular endothelial cells(HUVECs)injured by oxidized LDL(OX-LDL)and regulation on the expression of Ang1-Tie2,to explore its mechanism.MethodHUVECs was cultured in vitro and stimulated with OX-LDL(100mg/ml)for 24h,then treated separately with the serum contained different concentrations of DXR and PNSR1 decoction for 24h,then we observed the effects of DXR and PNSRI on its migration and tube formation.The content of vascular endothelial growth factor(VEGF)、Angiopoietin 1(Ang1)and Tie2 was detected by Western Blot.Chick Chorioallantoic Membrane was used to analyze the blood vessel formation in vitro.ApoE-/-mice were randomly divided into three groups feeding with high-cholesterol diet,treatment was continued for 12 weeks,then killed the mices.Tissue were selected and cuted,and IHC staining was performed by LSAB technique using mouse antihuman CD34 monoclonal antibody.Counterstaining was done by using hematoxylin.ResultDXR can inhibit the angiogenesis in vitro.DXR enhance the expression of Ang1,Tie2,decrease the expression of VEGF in HUVEC.The CD34 positive incidence is significantly lower in PNSR1 treated groups.ConclusionsDXR and PNSR1 can inhibit the angiogenesis in vitro.Such impact may be related to its regulating effect of Angl,Tie2,and VEGF in HUVEC.The CD34 positive incidence is significantly lower in PNSR1 treated groups.The results indicates PNSR1 alleviated the atherosclerotic plaque micro vessel density in the aortic larch in ApoE-/-mice.