| Objective:Since the concept of microns in traditional Chinese medicine was first proposed by Lu Fu-er,Chinese medicine is micron zed with its unique physical and chemical properties in the field of traditional Chinese medicine is widely used.However,many designs of Chinese medicine micron zed formulations are still not precise enough and depth.So,the quality of its powder standardization system has yet to be improved,especially the toxic ingredients of insect drugs.Therefore,to enhance the preparation of traditional Chinese medicine powder technology,standardization of quality standards for research,which is not only to develop Chinese medicine powder industry powder production and quality inspection standards provide a reference,but also will promote the development of the pharmaceutical industry.Our subject used cantharidin(traditional Chinese medicine with common poisonous)as research object,and we study the preparation process of cantharidin superfine powder and the physical and chemical characteristics and HPLC fingerprint of superfine powder of different particle size powders.It provides perfect test data for quality standard formulating superfine powder for traditional Chinese medicine industry.Methods:(1)The research on preparation technology of Mylabbis ultra-fine powder:The crushing time,temperature,water content,inventory and medium fill rate index were selected by using the D50 value measured and content determination.(2)The research on characterization,physical properties and quality control of Mylabbis ultra-fine powder:The appearance,microscopic structure,Physical characteristics,hygroscopic,TLC and control research of ultra-fine powder of Mylabbis were investigated.(3)The research on HPLC fingerprint of Mylabbis ultra-fine powder:Common model HPLC fingerprint were established as an index of its cancer cantharidin effective components.Results:(1)The optimization ultra-fine condition of Mylabbis was determined:water content was4%～6%,the feed was 150 g to 200 g,medium filling rate was 60%～70%,crushing time was 16 min advisable,crushing temperature was-10～10℃;the optimal particle size distribution was D90≤75μm.(2)The Characterization and evaluation results of different particle size of the Mylabbis powers showed that after Ultra-fine granting,the fluidity were deduced while the moisture absorption increased,but the moisture absorption were deduced when processed to ultra micro.Microscopic identification revealed that the ultra-fine powder had lost the microscopic identification of common features,and there was some broken organelles.The TLC results showed that the spot of coarse powder,fine powder.And super-fine powder is basically all the same while the most obvious spots are super-fine powder.(3)The specificity of HPLC fingerprint of Mylabbis ultra-fine power of 10 different areas was established,and was stronger.The HPLC chromatography of Mylabbis ordinary powder was consistent with that of ultra-fine power.9 common peaks were confirmed by using the gradient elution,similarity of 10 batches of samples were more than 0.90.Therefore,this fingerprint can controlling the quality of ultra-fine power and medicinal materials of Mylabbis in the same time.Conclusion:With the ultra-fine powder and ordinary powder of Mylabbis comparison,the appearance,the texture,the aerated density,the fluidity,the humidity absorption and the dissolution in vitro have greatly changed,however,major chemical compositions have not obviously varied.Besides,The HPLC result indicated that the content of cantharidin is much higher than that of Mylabbis ultra-fine powder,which indicates that granulation could increase the bioavailability.Besides,it may play an important role in saving the medicinal materials resources,And the HPLC fingerprint of Mylabbis ultra-fine power may be useful for providing the Experiment basis for controlling the quality of Mylabbis and the industry standard of ultra-fine powder. |
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