Research On Low Intensity Pulsed Ultrasound Stimulation Mesenchymal Stem Cell Transplantation To Treat Spinal Cord Injury

With the rapid development of modern transportation, building, industry, the number of patients with high-energy injuries in patients with traumatic proportion increases year by year, there is an upward trend in the incidence of Spinal cord injury. Spinal cord injury (SCI)is one of the field of orthopedic common diseases, SCI has the characteristics of high incidence, high disability and serious damage, the consequences could be disastrous, light who lost their ability to work, heavy can not be activities, urinary incontinence, loss of self-care ability. According to the statistical analysis of the SCI incidence of developed countries is 28.3 ?45 / one million/year,the incidence of 6.7 / one million/year. After the injury occurred in patients with neurological recovery rate is very low. So for the subsequent treatment of spinal cord injury, how to improve the cure rate is now one of the difficulties faced by the medical profession, but also one of the important research field.The current study has carried out a lot of spinal cord injury, but the main problem for repair are the neuron apoptosis, the poor regeneration ability of the axon and the damage caused by the formation of inhibitory micro-environment. And with the depth of the study,In particular, the results of cell transplantation studies show that, cell transplantation can promote the regeneration of the axon and myelin sheath, and then replace the apoptosis of nerve cells, thereby promoting the repair of spinal cord injury, and then for the recovery of sensory and motor function to create the conditions. For the treatment of spinal cord injury in cell transplantation, how to improve cell viability and differentiation is the key to successful transplantation. The present study shows that low intensity pulsed ultrasound(LIPUS) intervenes in seed cells to significantly improve cell proliferation and differentiation. However, how to select appropriate intervention parameter makes the best treatment is one of the problems. For multi - objective optimization, BP neural network based on genetic algorithm can obtain the optimal solution by global search. The experiment first set up a set of ultrasonic excitation system, including signal generator,amplifier, power supply and detection module. The amplifier module is built with THS4062 chip. Then through the medical experiments to draw the experimental results. Combining the BP neural network with the genetic algorithm, the experimental results were predicted by MATLAB software, and the best experimental conditions were obtained, namely, the combination of the optimal excitation parameters for the proliferation and activity of BMSCs. And verified the experiment to prove the correctness of the forecast results for the late transplant experiments to prepare.This article is based on the biological effects of low intensity pulsed ultrasound on bone marrow mesenchymal stem cells, cells were stimulated with different parameters of LIPUS and predicted by BP neural network model based on genetic algorithm. The experimental results were verified by experiments on the experimental conditions. The results showed that the cell viability was 24.9% higher than that of the control group, and the cell activity was better than the other Under the pre-determined experimental conditions.