| BackgroundFunctional dyspepsia(functional dyspepsia,FD)is a common and frequent clinical disorder of gastrointestinal function,main show is Postprandial full bilge unwell,early enough,upper abdominal Pain and a burning sensation in the upper abdomen,according to the Roman IV divided into discomfort after eating syndrome(Postprandial Distress syndrome,PDS)and upper abdominal Pain syndrome(Epigastric Pain syndrome,EPS),the Postprandial discomfort syndrome mainly for full and Postprandial fullness,early on abdominal Pain syndrome are mainly located in the upper abdomen Pain or a burning sensation.In the global incidence of 11.5%～29.2%[1],and had an impact on the world’s more than a quarter of the population.Its pathophysiological mechanism is not very clear,at present,a lot of western medicine experts think increased and abnormal gastrointestinal dynamics,visceral sensitivity,excessive gastric acid secretion,helicobacter pylori infection and brain-im axis,autonomic nervous system and gastrointestinal hormone and social psychology factors,mainly by promoting gastrointestinal dynamics,to reduce the internal high sensitive,treatment of HP infection,inhibiting gastric acid secretion,protect the gastric mucosa and mental psychotherapy,use single targets more symptomatic treatment.TCM holds that functional dyspepsia epigastric burning sensation in the main with the corresponding "noisy","on abdominal pain" and "stomach","weiwantong",and after a meal is not to early full main accordingly with the "new" full,is the basic pathogenesis of spleen,dysfunctional,stomach indicates lag,gas is given priority to with qi and pixu(spleen deficient),treatment is given priority to with down-regulated,traditional Chinese medicine has its unique advantages in the treatment of FD,this study by adopting the spleen prescription intervention by way of in vivo and in vitro FD spleen deficiency rat model of combined disease and study its mechanism.Purpose1.The number of I pixu(spleen deficient)party and amomum 6 gentleman soup in vivo pharmacokinetics and pharmacological experiments,observation spleen and prescription of FD spleen deficiency model rats gastrointestinal function combined disease,discuss the possible mechanism for the treatment of FD;2.By in vitro condition FD spleen deficiency model rats of different dose combined disease and spleen and prescription to intervene,discuss spleen and prescription of different parts of the stomach tissue of rat gastric dynamic directly influence;3.Observe the in vitro under different spleen and stomach tissue at different positions of the combination of dynamic effect directly.ResearchExperiment 1,FD spleen deficiency rat model establishment and evaluation of combined diseaseObjective:The establishment and evaluation of FD spleen deficiency rats combined disease modelMaterials and methods:Will be 30 male SD rats(rats)were randomly divided into normal group,the pure iodine acetamide lavage group(disease),iodine acetamide lavage superposition small platform standing group(group)combined disease,born 7 purchases,feeding adaptability to 3 days.Born 10 SD male rats,normal group daily with 2%sucrose solution to fill the stomach;Model group daily lavage with 0.1%iodine acetamide solution,each only 0.2 ml,continuous lavage 6 days;When rats were 3 weeks,excluding females,cage,to rats after six weeks,continue to feed to normal mice;Iodine acetamide superposition of small platform standing daily 18:00-8:00 to small platform to stand,for 14 consecutive days.Building were observed after the general condition,body weight,food intake,water quantity,water holding power,sugar preference,serum lactic acid,D-xylose,amylase content,etc.Results(1)general situation:model group combined disease rat body weight,food intake,water volume compared with normal group and the other model group were significantly lower(P<0.05).(2)determination of holding power:the model group rats holding power combined disease compared with normal group and the other model group decreased significantly(P<0.05).(3)sugar water preference:model group rats combined disease in sugar water consumption has decreased,but compared with the normal group and the other model group,there was no statistically significant difference(P>0.05).(4)the serological indexes:compared with normal group and the other model group,model group combined disease and serum D-xylose and amylase content decreased significantly(P<0.05),serum lactic acid content increased significantly(P<0.01).Experiment 2,spleen and prescription of FD spleen deficiency model of rats in vivo combined disease and gastrointestinal dynamics mechanism studyObjective:By observing Sand observation I pixu(spleen deficient),party and fragrant 6 gentleman soup of FD model of spleen deficiency rats gastrointestinal function,from the gastric emptying and small intestinal propulsion and serum gastrointestinal hormone levels to explore its possible mechanism for the treatment of FD.Materials and methods:Taking iodine acetamide superposition xiaoping legislation to establish the station FD spleen deficiency rat model of combined disease,the experimental animals were randomly divided into normal group,model group,the western medicine group(Pan Li ketone group),I pixu(spleen deficient),high dose group,middle dose group,low dose group,sweet sand 6 gentleman high dose group,middle dose group,low dose group.End of the building,per 10 ml,1 kg_weight of rats dosing/1 ml to I pixu(spleen deficient)or sweet sand 6 gentleman or more Pan Li ketone or distilled water to fill the stomach 14 d.In the process,regularly observed and recorded in the rat body weight,food intake,water content,holding power,such as color,activity generally.After completion of treatment,given to rats by gastric nutritional semisolid paste,calculate each rat gastric retention rate and small intestinal propulsion in ratio.Take blood anesthesia ventral aorta,with ELISA or colorimetry to detect serum.amylase,D-xylose,lactic acid,the stomach(MTL),gastric gastrin-releasing(GAS),promote the auxin(Ghrelin)and the gallbladder contraction,(CCK)and somatostatin(SS),vasoactive intestinal peptide(VIP)content.Results1 General ConditionBefore the treatment,compared with normal group,model group and each group of rats body weight,food intake,water content,holding power were significantly lower(P<0.05).Compared with model group,in addition to the normal group,the rest between each rat body weight,food intake,water content,holding power had no obvious difference(P>0.05).After treatment,compared with model group,western medicine group,the sand is low,medium and high dose group,pixu(spleen deficient),high dose group of rats body weight was significantly increased(P<0.05).Sand high dose group,high dose group of rats drinking pixu(spleen deficient)significantly increased(P<0.05).Sand high,medium and low dose group,high,medium and low dose group of pixu(spleen deficient)significantly increased food intake in rats(P<0.05).Western medicine group,and sand high,middle dose group,high and low dose group of pixu(spleen deficient)holding power of rats significantly increased(P<0.05).Compared with western medicine group,sand high dose group,high dose group of rats drinking pixu(spleen deficient)significantly increased(P<0.05).High,middle dose group of pixu(spleen deficient)rats significantly increased food intake(P<0.05).Each rat body weight,holding power difference compared with the western medicine group had no statistical significance(P>0.05).2 Stomach retention rate and small intestine propulsion(1)compared with normal group,model group rats gastric residual rate is significantly higher than in normal group(P<0.01);Compared with model group,western medicine group,low dose pixu(spleen deficient),sweet sand high dose group of rats gastric residual rate significantly decreased(P<0.05);Compared with western medicine group,sand of pixu(spleen deficient),high dose group,low,middle dose group of rats with gastric residual rate is significantly higher than in western medicine group(P<0.05).(2)compared with normal group,model group in the rat small intestine propulsion ratio was significantly lower than normal group(P<0.05);Compared with model group,western medicine group,the low,middle and high dose group of pixu(spleen deficient),sand high dose group of small intestine propulsion ratio significantly increased(P<0.05);Compared with western medicine group,high dose group of small intestine propulsion pixu(spleen deficient)were significantly higher than western medicine group(P<0.01),and sand low doses of small intestine propulsion ratio was significantly lower than western medicine group(P<0.05).3 Serological Indicator(1)compared with normal group,model group,the serum D-xylose content was significantly lower than normal group(P<0.05);Compared with model group,western medicine group,low dose group of serum D xylose content pixu(spleen deficient)were significantly higher than that of model group(P<0.01);Compared with western medicine group,the serum D-xylose content differences between traditional Chinese medicine group were not significant(P>0.05).(2)compared with normal group,model group was significantly reduce serum amylase content(P<0.05);Compared with model group,western medicine group,high dose group,pixu(spleen deficient)and the sand is low,serum amylase content in high dose group were significantly higher than that of model group(P<0.05);Compared with western medicine group,the serum amylase content differences between traditional Chinese medicine group were not significant(P>0.05).(3)compared with normal group,model group significantly increased serum lactic acid content in the model group(P<0.05);Compared with model group,western medicine group,after the treatment of pixu(spleen deficient),high dose,the sand is low,the dosage of serum lactic acid levels were significantly lower than model group(P<0.05);Compared with western medicine group,the serum lactic acid content differences between traditional Chinese medicine group were not significant(P>0.05).(4)compared with normal group,model group serum gastric dynamic content was significantly lower than normal group(P<0.01);Compared with model group,western medicine group,the low,middle and high dose group of pixu(spleen deficient),the sand is low,the serum gastric dynamic content,high dose group were higher than that of model group(P<0.01);Compared with western medicine group,sand of pixu(spleen deficient),high dose group,low serum gastric dynamic content,middle dose group were higher than that of western medicine group(P<0.05).(5)compared with normal group,model group serum gastric secrete element content is significantly lower than normal group(P<0.01);Compared with model group,western medicine group,the low,middle and high dose group of pixu(spleen deficient),incense in the sand,low serum gastric secrete element content,high dose group were higher than that of model group(P<0.01);Compared with western medicine group,high dose group of pixu(spleen deficient)serum gastric secrete element content is significantly higher than western medicine group(P<0.05).(6)compared with normal group,model group and promote gastric serum growth hormone levels significantly lower than normal group(P<0.05);Compared with model group,western medicine group,high dose group,pixu(spleen deficient)and sweet sand high dose group had a significantly higher serum promote stomach auxin content model group(P<0.05);Compared with western medicine group,the sand high dose group,high dose group of serum pixu(spleen deficient)and promote gastric auxin content is significantly higher than western medicine group(P<0.05).(7)compared with normal group,model group serum gallbladder contraction content higher than the normal group,but there was no statistically significant difference(P>0.05);Compared with model group,amomum low dose group of gallbladder contraction content of serum was significantly lower than model group(P<0.05);Compared with western medicine group,incense sand high dose group had a significantly higher serum levels of gallbladder contraction content of western medicine group(P<0.05)(8)compared with normal group,model group had a significantly higher serum levels of vasoactive intestinal peptide content in normal group(P<0.05);Compared with model group,incense sand high dose group was significantly higher than that of model group(P<0.05);Compared with western medicine group,the serum levels of vasoactive intestinal peptide content differences Chinese medicine group were no statistical significance(P>0.05)(9)compared with normal group,model group,serum levels of somatostatin content is significantly higher than normal group(P<0.05);Compared with model group,amomum dose group of serum somatostatin content decreased significantly(P<0.05);Compared with western medicine group,the serum levels of somatostatin content differences Chinese medicine group were no statistical significance(P>0.05)Experiment 3,spleen and prescription of FD model of spleen deficiency rats in vitro tissue the effect of gastric dynamics researchObjective:By observing the Ⅰ pixu(spleen deficient),party and amomum 6 gentleman soup intervention in functional dyspepsia model of spleen deficiency rats in vitro gastric tissue,from the amplitude and frequency of gastric body,gastric antrum and the tension of stomach bottom Angle,trying to clear Ⅰ pixu(spleen deficient),party and amomum 6 gentleman decoction in vitro gastric dynamic mechanism of action of adjustment.Materials and methods:Take iodine acetamide superposition method of small platform to build functional dyspepsia spleen deficiency rat model of experimental animals were divided into normal group,model group and normal group and model group building after rat gastric body,gastric,gastric antrum,different tissue is each part of the model group and normal group differences in shrinkage,in vitro to different concentrations of I pixu(spleen deficient),party and sand 6 gentleman soup,observation model to amplitude and frequency of gastric body,gastric antrum and the impact of stomach bottom tension.Results:(1)in vitro condition,model of rat stomach bottom tension was significantly higher than that of normal group,the difference was statistically significant(P<0.05),the model rats amplitude and frequency of gastric antrum and the gastric body were significantly lower than normal group,the difference was statistically significant(P<0.05).(2)to give different concentrations of pixu(spleen deficient)I party mediated by FD model rats stomach bottom tension increased with the increase of concentration,the difference was statistically significant(P<0.05),while incense sand 6 gentleman stomach bottom tension decreases with drug concentration,and the difference was statistically significant(P<0.05).(3)provide amplitude with concentration and dosage of gastric antrum after enhanced obviously,the difference was statistically significant(P<0.05),the contraction frequency no significant change(P>0.05),give sweet sand 6 gentleman when low concentration can significantly increase the amplitude and frequency of gastric antrum(P<0.05),when the concentration reaches a certain dosage is inhibition of gastric antrum contraction frequency and amplitude(P>0.05).(4)after gastric body contraction amplitude decreases with drug concentration,the difference was statistically significant(P<0.05),the contraction frequency is increased,the difference was statistically significant(P<0.05),give sweet sand 6 gentleman amplitude and frequency of gastric body when different concentrations were significantly decreased,the difference was statistically significant(P<0.05),when the concentration reaches a certain dosage is inhibition of gastric antrum contraction frequency and amplitude(P<0.05).Experiment 4,different spleen drug combinations on the function of normal rats in vitro gastric dynamic researchObjective:By observing different spleen medicine on in vitro amplitude and tension of stomach bottom of gastric antrum of rats,to explore in vitro under different spleen medicine for stomach dynamic adjustment.Materials and methods:Experimental animals can be divided into four groups,taking gastric antrum of rats,stomach bottom different organizations,in vitro to different concentrations of spleen medicine,medicine of down-regulated,spleen wet medicine and traditional Chinese medicine drug combinations,observation on amplitude and the role of stomach bottom tension difference of gastric antrum.Results:(1)traditional Chinese medicine spleen,spleen wet group and can significantly improve the tension of stomach bottom in the group,the difference was statistically significant(P<0.05),down-regulated group can significantly reduce the tension of stomach bottom,the difference was statistically significant(P<0.05).(2)Chinese medicine spleen group,spleen wet group can increase gastric antrum contraction,the difference was statistically significant(P<0.05),down-regulated group can significantly reduce the distal gastric contraction,the difference was statistically significant(P<0.05),group of gastric antrum in spleen temperature effects have no difference(P>0.05).Conclusion1.Iodine acetamide lavage superposition method improved small platform standing to make animal model in line with the characteristics of spleen deficiency,functional dyspepsia with gastric dynamic disorders.2.The spleen prescription can improve FD rats gastrointestinal absorption function and lactic acid accumulation caused by limb tired fatigue state.3.The spleen prescription can directly promote the gastric emptying and small intestinal propulsion in rat models or by improving the gastrointestinal hormone,adjust gastric bowel movement,among which side of the small intestine propulsion function I pixu(spleen deficient)is superior to the sand 6 gentleman.4.In the condition of in vitro,spleen and prescription can be directly ACTS on the stomach tissue and improving stomach,spleen and spleen medicine prescriptions to exert its from different angles of gastrointestinal two-way adjustment function,and present a dose-dependent. |
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