Profiling The Dynamic Of PBMC Immune-related Indicators Of Lung Cancer After 5 Periods Chemotherapy

BackgroundLung cancer has become the highest incidence and mortality of malignant tumors in the world.With the development of molecular immunology,immunotherapy has become one of the most important treatments after surgery,chemotherapy and radiotherapy.The combination of chemotherapy and immunotherapy could enhance the antigenicity of tumor cells and the activity of immune cells.However,immune function is related to the drug,dosage and different administration methods in the course of chemotherapy,in addition to the differences of immune function of different individuals,the immune function would be more complex and changeable in the treatment.The optimal time for combination of chemotherapy and immunotherapy still need to be studied.ObjectiveProfiling the dynamic changes of peripheral blood lymphocyte subsets,checkpoints molecules of T lymphocyte,cytokines and other indicators of lung cancer after 5 periods chemotherapy.Thereby observing the changes of immune state is conductive to evaluate the systemic immune function and spy upon the tendency of immune function.Aim to explore the optimal time for the combination between chemotherapy and immunotherapy,and provide experimental basis for immunotherapy intervention.MethodsCounted the number of T lymphocyte subsets,B lymphocytes and NK lymphocytes,and detected the expression of T lymphocyte co-stimulatory molecule and cytokines in peripheral blood by flow cytometry.Peripheral blood samples were taken before chemotherapy.Meanwhile,collected the clinical datum of total patients.To analyze the tendency of such indicators from first to the fifth chemotherapy.Flowjo 7.6.1 was used to analyze the flow charts and data were analyzed by SPSS 20.0 statistical software.All measurement data were analyzed by???+s,the comparison between the two groups using independent sample t test,?=0.05 as tests,with P<0.05 for the difference was statistical significant.Results1. Analysis of 23 patients with lung cancer,the statistical showed that the number of leukocytes,neutrophils,lymphocytes,platelets and hemoglobin decreased significantly;2.Blood biochemical(total protein,albumin,globulin,alanine aminotransferase,aspartate aminotransferase,glucose)index did not change significantly;3.The number of CD8~+T lymphocytes,CD19~+B lymphocytes and CD16~+CD56~+NK cells were decreased,and the number of CD4~+T lymphocytes were significantly increased;4.The PD-1,CTLA-4 and CCR-4 expression of CD8+T lymphocytes were up-regulated,the PD-L1 expression was down-regulated,and there was no significant difference in the expression of CD137 and LAG-3;5.The PD-1 expression of CD4~+T lymphocytes was up-regulated,the PD-L1expression was down-regulated,and the expression of CTLA-4,LAG-3,CCR-4 and CD137 was not changed significant;6.The content of peripheral blood cytokines(IL-2,IFN-?,IL-4,IL-6,IL-10,TNF-?and IL-17A)showed no significant difference in the whole periods of chemotherapy.ConclusionIn the course of multicycle chemotherapy for patients with lung cancer,there is a dynamic decreased of the number of CD8~+T lymphocytes,CD19~+B lymphocytes,CD16~+CD56~+NK cells and increased of CD4~+T lymphocytes.The co-stimulatory molecule expression of PD-1,CTLA-4 and CCR-4 with T lymphocytes were up-regulated.Profiling these indicators is of great significance to the combination treatment of chemotherapy and immunotherapy.