Study On Chromatographic Fingerprint Of Tripterygium Wilfordii Hook.f And Tripterygium Preparations By HPLC

Tripterygium wilfordii is one of traditional Chinese medicine.It is widely used for the treatment of rheumatoid arthritis,lupus erythematosus,glomerulonephritis,kidney disease,psoriasis,etc.The chemical composition of Tripterygium wilfordii is complex,already more than 100 kinds of compounds were separated and identified from it,mainly half terpenoids(including half sesquiterpanes alkaloids),two terpenoids,triterpene compounds.Tripterygium glucosides tablets were made from extract of debark root of tripterygium wilfordii by column chromatography.Tripterygium tablets were made from ethyl acetate extract of tripterygium wilfordii root.It is not enough to whole control the quality of the preparations only by a certain kind of active ingredient or index component content determination,due to its complicated chemical composition and high toxicity.In view of the complexity of the chemical composition of tripterygium wilfordii,we used neutral alumina solid phase extraction small column to treat samples,respectively established the fingerprint chromatogram of Tripterygium wilfordii Hook.f and tripterygium tablets.Because the acid composition will be absorbed by neutral alumina,we used MAX solid phase extraction small column to process the samples and respectively established the fingerprint to characterize the acidic two terpenoids,triterpene compounds.The methods are stable,accurate,repeatable and can be applied to control the quality of Tripterygium wilfordii Hook.f and tripterygium preparations.The results showed that in the same tripterygium wilfordii plant,kinds of ingredients and contents in the root bark were significantly greater than in the debark root,stems and leaves.Different plants from the same producing area also have certain differences.Different batches of tripterygium preparations from the same pharmaceutical company has good similarity,production process is relatively stable,except Xin company.Different batches from different pharmaceutical companies have difference in the similarity.Clustering analysis showed that,tripterygium glucosides tables from 10 different companies can get together to a categories and separated from the Tripterygium tablets.But the tripterygium glucosides categories has kinds of multi-level,showed that different companies the consistency is poor.Neutral composition fingerprint study found that Bin company was separated from others,is quite different.Acid composition fingerprint study found that Ji company is separated from others,have certain differences with other tripterygium glucosides tablets companies.According to the LC-MS analysis and the tripterygium wilfordii reference substance determine the peak ownership,we found the main components are terpenoids and alkaloids.The contents of chemical components of tripterygium glucosides tablets from 10 different companies have significant difference.As national standards only control a single component,different enterprise sample has different in clinical medical efficacy and safety.As tripterygium preparations have narrow therapeutic window and high toxicity,the quality standards can't guarantee the safety and effectivety of.clinical drug therapy.In this paper the established fingerprints can evaluate the quality of Tripterygium wilfordii Hook.F and tripterygium preparations in general.They can provide support for the rational use of medicinal materials and meet the clinical safety of tripterygium wilfordii medication.