The Protect Effect And Mechanism Of Ginkgo Biloba Extract L On Central Nervous Toxicity Of Acrylamide

Objective: Acrylamide is a moderately toxic chemicals which people contact widely in daily life.It not only will injury peripheral nerve,but also the central nervous system when ACR progresses in the human body.Ginkgo biloba extract L,a traditional Chinese medicine,has significant protective effect in Cardiovascular and central nervous system.Previous studies revealed neuroprotective effects of EGb through antioxidation.However,whether or not EGb could be used to treat neuroprotection induced by acrylamide(ACR)gavage administration.It was aimed to investigate the protective and mechanism of Ginkgo biloba extract L against acrylamide neurotoxicity.Methods: The KM mice were rando m Ly divided into five grous of eight mice each: Control group-did not undergo any procedure;ACR group-gavage administrated saline in the morning and 20 mg/kg/d ACR in the afternoon;30 mg/kg group-gavage administrated 30 mg/kg/d EGb in the morning and 20 mg/kg/d ACR in the afternoon;60 mg/kg groupgavage administrated 60 mg/kg/d EGb in the morning and 20 mg/kg/d ACR in the afternoon;120 mg/kg group-gavage administrated 120 mg/kg/d EGb in the morning and 20 mg/kg/d ACR in the afternoon.Applications were performed four weeks.Gait score evaluated 3 days before euthanized.The open-field test was performed 3 days on mice before euthanized,walked through grid numbers and standed numbers were recorded in 5 minutes.The expression of DCX,BDNF and GAP-43 were detected with immunohistochemistry in mice hippocampus.The expression of DCX,BDNF and GAP-43 were detected with Western Blot in mice hippocampus.Results: 1、 Mice lack of force to bear body weight in ACR group and can not stand;Compare with ACR group,the mice in EGb 30 mg/kg,EGb 60 mg/kg and EGb 120 mg/kg groups had improved the force to bear body weight,can stand in short time periods.2、 Ginkgo biloba extract L can ameliorate the nervous symtoms of foot weakness,obvious movement abnormalities characterized on outreach of legs obviously by ACR poisoning in mice(P<0.05).3、 In open-field test,more went through grid numbers in control group than ACR group(P<0.01)in five minutes;EGb 30 mg/kg and EGb 60 mg/kg groups more than ACR group(P<0.05).In stand numbers,ACR group less than control group in five minutes(P<0.01);EGb 60 mg/kg groups more than ACR group(P<0.05);but there were no significant differences between EGb 30 mg/kg、EGb 120 mg/kg groups and control group(P(29)0.05).4、 Results of immunohistochemistry: the expression of DCX and GAP-43 in ACR group less than control group(P<0.05);and there were significantly increased in EGb 30 mg/kg,EGb 60 mg/kg and EGb 120 mg/kg groups compare with ACR groups(P<0.05).5、Results of Western Blot: the expression of DCX,BDNF and GAP-43 in ACR group mice of hippocampus less than control group(P<0.05);and the expression increasesd in EGb 60 mg/kg and EGb 120 mg/kg groups compared with ACR group(P<0.05).And the the expression of BDNF in EGb 30 mg/kg,EGb 60 mg/kg and EGb 120 mg/kg groups increased compare with ACR group(P<0.05).Conclusions: 1 、 Ginkgo biloba extract L can ameliorate the neurotoxicity of acrylamide,and enhance mice athletic capability.2、Ginkgo biloba extract L can repair the ACR injury by increasing the expression of DCX,BDNF and GAP-43 in mice hippocampus.3、The study might provide science foundation for Ginkgo biloba extract L to prevent and treat neurotoxicity of acrylamide.