Effect Of Emodin On The Expression Of TLR4,NF-κB,IL-6, In Kidneys Of Diabetic Rats

Objective: It is to observe the effect of Traditional Chinese medicine Emodin on the expression of TLR4,NF-κB and IL-6 in the kidneys of diabetic rats,and the protective effects of Emodin on the diabetes renal tissue were examined in this study.The mechanism of its prevention on diabetic kidneys was explored to provide theoretical basis for clinical application.Methods: 50 adult male Wistar rats were randomly divided into two groups: normal control group(NC,n=10)and diabetes mellitus(DM)group(n=40).The rats in DM group were injected STZ 60 mg/kg one-time into enterocoelia(STZ before use with 0.1 M with the concentration of 1% sodium citrate buffer solution,p H=4.2)and the rats in NC group were injected same volume of sodium citrate buffer.After 72 hours,the rats whose blood glucose≥16.7mmol/L had chosen as diabetic rats.Diabetes rats were randomly divided into two groups: DM and Emodin intervention(ER)group(n=10).The rats in ER group was given the 100mg/(kg body weight)Emodin with intragastric administration.The rats in NC and DM groups have given the same volume of saline.All of rats drink and eat freely in the experiment.After 8 weeks,The pentobarbital anesthesia with 80 mg/kg used in rats to harvest blood and renal tissue.5-6m L blood was taken from renal for serum blood sugar,TC,TG,ALT,AST,Cr,BUN.The renal fixed with 4% paraformaldehyde for immunohistochemistry to known the pathological changes.The positive and integrated expression of TLR4,NF-ΚB and IL-6 on renal tissue were examined with immunohistochemical staining.The measurement data were described by (?)±S.The comparison of each group before and after intervention using matching t test.The one-way ANOVA used for test the difference between each group.Kruskal-Wallis H test is used when variance is not neat.All the data were processed with SPSS 21.0 statistical processing software and inspection level: α=0.05.Results:1 The influence of the general condition of ratsThe rats of DM group and ER group compared with NC group: mental state is poor,water quantity increased,urine volume increased,the colour dark matt,unresponsive,means less still.2 The influence on blood glucoseBefore the treatment,DM group and ER group were obvious higer than NC group(P<0.05);DM group and ER group compared with each other has no obvious difference(P>0.05).After the treatment,DM group and ER group were obvious higer than NC group(P<0.05);DM group and ER groups compared with each other has no obvious difference(P>0.05).There were no difference in blood glucose before and after the treatment of each group.3 The influence on total cholesteol and triglycerideAfter the treatment,compared with NC group,DM group and ER group total cholesterol and triglyceride have no obvious difference(P>0.05);DM and ER group compared with each other have no obvious difference(P>0.05).4 The influence on ALT,AST,Cr,BUNAfter the treatment,compared with NC group,there were no obvious difference in ALT,AST,Cr,BUN of each group(P>0.05).5 The influence of the expression of TLR4After lavage treatment for 8 weeks,compared with NC group,the expression of TLR4 in DM group and ER group significantly increased(P<0.05);Compared with DM group,the expression of TLR4 in ER group significantly decreased(P<0.05).6 The influence of the expression of NF-κBAfter lavage treatment for 8 weeks,compared with NC group,the expression of NF-κB in DM group and ER group significantly increased(P<0.05);Compared with DM group,the expression of NF-κB in ER group significantly decreased(P<0.05).7 The influence of the expression of IL-6After lavage treatment for 8 weeks,compared with NC group,the expression of IL-6 in DM group and ER group significantly increased(P<0.05);Compared with DM group,ER group significantly decreased(P<0.05).Conclusion:1 The expression of TLR4,NF-κB and IL-6 are increased in NC group rats’ renal tissue,suggesting that TLR4,NF-ΚB and IL-6 play important roles in the development of diabetic renal lesions.2 Emodin inhibit the expression of TLR4,NF-κB and IL-6 in renal tissue of diabetes rats,indicating that it can protect the renal tissue of diabetes rats.3 Emodin have no influence on glucose,total cholesterol and triglyceride of diabetes rats,suggesting that emodin’s protection of kidneys independent the help of glucose,cholesterol and triglyceride.So it has a certain therapeutic effect on diabetic nephropathy immediatly.4 The results showed that emodin has no influence on rats’ ALT,AST,Cr and BUN,when the dosage of emodin was 100mg/kg,it comes the treatment effect above.