| AIM: Previous studies have reported that ginsenoside Rd(G-Rd)exhibit antiinflammatory properties and neuroprotective effects.However,the effect of G-Rd on anti-inflammatory properties of the intestinal epithelial cell has not been reported at present.The aim of the present study was to investigate the inhibitory effects and the mechanisms of G-Rd on lipopolysaccharide(LPS)-induced inflammatory responses in HT-29 human colon epithelial cell.Methods:The HT-29 cells were treated with LPS(1μg/ml)with or without GRd(50μM,100μM and 200μM).The expression of inflammatory cytokines il-8蛋白was measured by Enzyme-linked immunosorbent assay(ELISA);Real timequantitative polymerase chain reaction(RT-qPCR)was used to analyze the messenger RNA(mRNA)levels of inflammatory cytokines IL-8.The regulation of ginsenoside Rd on NF-κB pathway was determined by Western blotting(WB),immunohistochemistry(IHC)and Electrophoretic mobility shift assay(EMSA).Results: 1.RT-qPCR assays showed that G-Rd significantly suppressed the LPS induced IL-8 mRNA expression.ELISA assays showed the secretion of IL-8 protein upon LPS stimulation was also attenuated by G-Rd.2.Western blotting,immunohistochemistry and electrophoretic mobility shift assay results showed that ginsenoside Rd significantly suppressed LPS-induced NF-κB activity.Conclusion: Ginsenoside Rd could suppress the expression of IL-8 in LPSinduced HT-29 cells via downregulating the NF-κB pathway activity,suggesting its potential application in inflammatory bowel disease(IBD)therapy. |
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