Alprostadil Injection Mitigates Lung Ischemia And Reperfusion Injury Via Suppressing Autophagy

Objective:To explore the changes in autophagy levels and the protective effect of alprostadil injection in rat lung ischemia and reperfusion injury(IRI),and further study to discuss whether the alprostadil injection could mitigate lung ischemia and reperfusion injury via regulating autophagy.Methods:Rats lung ischemia-reperfusion(I/R)model was established by clamping of the right hilum thoracotomy.A total of 75 rats were randomly divided into five groups(n=15):sham-operation group(Sham),ischemia and reperfusion group(I/R),alprostadil injection conditioning group(Alp),alprostadil injection and rapamycin conditioning group(Alp+Rap)and rapamycin conditioning group(Rap).After 60 min of ischemia and120 min of reperfusion,the rats were executed.Blood gas analysis,the wet/dry weight ratio(W/D)of lung,histopathologic scoring of lung tissue and autophagy markers(Beclin-1 and LC3)were compared.Results:Compared with sham group,the levels of PaO2 significantly decreased in I/R group,the W/D values significantly increased,the degree of lung injury aggravated significantly and the expression of autophagy markers(Beclin-1 and LC3)was excessively activated.Compared with I/R group,the levels of PaO2 significantly increased in Alp group,the W/D values significantly decreased,the degree of lung injury reducedsignificantly and the expression of autophagy markers(Beclin-1 and LC3)was excessively depressed.The autophagy activator,and the Rapamycin could weaken the effect of alprostadil injection.Conclusion:The level of autophagy is increased in lung ischemia reperfusion injury.Alprostadil injection could mitigates lung ischemia and reperfusion injury via suppressing autophagy,and the activation of autophagy could aggravate the lung ischemia and reperfusion injury.