Rosuvastatin Reduces Lung Ischemia-Reperfusion Injury By Inhibiting Hyperactivated Autophagy

Objective:To investigate the changes of autophagy in lung tissue of rats after ischemia-reperfusion injury and the protective effect of rosuvastatin preconditioning on lung ischemia-reperfusion injury.To further elucidate whether rosuvastatin can alleviate lung ischemia-reperfusion injury in rats by regulating hyperactivated autophagy.Methods:40 male adult SD rats were selected.After opening the chest,the small rib c age was used to open the chest,expose the lung tissue,free the right lower lung ligament and the right hilum,and clamp the right hilum with a non-invasive vas cular clamp.LIRI model.Forty rats were numbered and divided into sham operati on group,ischemia/reperfusion（I/R）group,rosuvastatin（ROS）group and rapamyc in group（RAP）by random number table method.And rosuvastatin pretreatment+rapamycin（ROS+RAP）group and other 5 groups,each group of 8 first clip t he right hilar for 1 h,then release the right hilar reperfusion for 2 h after the th oracic Arterial blood gas analysis needles were collected and the rats in each gro up were sacrificed and the right lung tissue was taken out.Then,the arterial bloo d gas analysis value,lung tissue wet/dry weight ratio（W/D）and HE staining wer e used to observe the pathological changes of lung tissue.The expression of Becl in-1 and LC3 in lung tissue was determined by Western blot and immunohistoche mistry.Results:Compared with the Sham group,the PaO₂ value of the I/R group decreased,the PaCO₂ value increased,the PH value decreased,the wet-dry weight ratio incre ased significantly,the alveolar structure was significantly impaired,edema,marked hyperemia and inflammatory cells were observed,Beclin-1,The expression of LC3-II was significantly increased.Compared with the I/R group,the PaO₂ value of the ROS group increased,the PaCO₂ value decreased,the pH value increased,th e wet-to-dry weight ratio decreased significantly,and the alveolar cavity showed mild edema and congestion,showing a small amount of inflammatory cell infiltrat ion,Beclin.-1,LC3-II expression was significantly reduced.Compared with the I/R group,the PaO₂ value of the RAP group decreased,the PaCO₂ value increased,the pH value decreased,the wet-dry weight ratio increased significantly,and a la rge amount of congestion,edema,and alveolar cavity structure were significantly i mpaired in the alveolar space.Beclin-1 The expression of LC3-II was significantl y increased.Compared with the ROS group,the PaO₂ value of the ROS+RAP gr oup decreased,the PaCO₂ value increased,the PH value decreased,the wet-dry w eight ratio increased significantly,a large amount of congestion and edema in the alveolar cavity,and alveolar structure were significantly impaired,Beclin-1,LC3-II expression was significantly increased.Conclusion:Rosuvastatin pretreatment can significantly reduce lung ischemia-reperfusion in jury to provide lung protection,which may be through inhibition of hyper-activate d autophagy;providing new ideas for clinical treatment or drug intervention to all eviate ischemia-reperfusion injury.