Immune Related Genes Expressed In HIV/AIDS In Hot And Humid Intrinsic Certificate Studies

Objective :Using gene chip technology to detect HIV/AIDS hot and humid card of intrinsic immune gene expression;Select related to HIV/AIDS hot and humid card of intrinsic immune mRNA;Using the data mining technology to find immune m RNA for data analysis,analysis the function of immune related m RNA and signaling pathways,and hot and humid embodiment of HIV/AIDS and immune related m RNA and signaling pathways for the relationship between research,explore the immune m RNA in HIV/AIDS the molecular mechanism in the hot and humid intrinsic card,find the HIV/AIDS hot and humid embodiment the specificity of m RNA expression,to clarify the AIDS in molecular mechanism of syndromes and syndrome differentiation of traditional Chinese medicine treatment of AIDS objective to provide experimental basis and the reference,provide technical support for AIDS research platform of traditional Chinese medicine.Methods1.This topic research object is divided into 16 patients with HIV/AIDS hot and humid embodiment card,health control group 20 cases,from a region of henan province.AIDS hot and humid embodiment certificate by the state ministry of health,2011 patients with HIV/AIDS diagnosis and treatment guidelines and national project prior to the project team achievements "HIV/AIDS diagnosis often witness scale" for diagnostic criteria.2.Collect patients and normal control group in the morning on an empty stomach peripheral blood as the research sample,total RNA extracted samples were studied.Application AFFX Human Genome U133 Plus 2.0 chip expression of m RNA expression Detection,the use of BRB Array Tools software(Version 4.1),the Percent of data missing or filtered out exceeds 50%,the Percent of absent(i.e.for,DetectionCall = A)data exceeds 50% of the standard for all kinds of gene chip data for analysis.3.Using human immune gene chip to screen hot and humid embodiment syndrome related gene expression.Multiples(1)according to the p < 0.05,differences between FC > 2 or FC 0.5 standard screening or less damp and hot intrinsic contrast group and healthy control group difference of immune genes;(2)enter http://david.abcc.ncifcrf.gov/summary.jsp online analysis of body function;(3)to filter out the immune genetic variations of input KEGG database,find out the specific expression of genes involved in signaling pathways and immune differences were analyzed.(3)application of Internet tool STRING(http://string.embl.de),the expression of specific differences in genetic relationship analysis.Results :1.SPSS21.0 analysis,two groups no significant differences in age,gender composition.2.Screening compared the two groups of immune genetic differences associated with77,according to the gene ontology function,genetic variations associated with screening immune response process article 20: CXCL1,FOS,NFKBIZ,CEBPB,IL8,CX3CR1,IL1 B,CLEC7A,CCL4,SOD2,PLAUR,NFKBIA,PTGS2,SOCS3,TRIB1,TREM1,NFIL3,FTH1,EGR1,JMJD6;3.Found in the database KEGG_PATHWAY HIV/AIDS in the hot and humid card of intrinsic immune related genes,involving IL-17 signaling pathway have 6,TNF signaling pathway have 6,NF-kappa B signaling pathway has five,Toll-like receptor signaling has five,Pathways in cancer has five,etc.4.The HIV/AIDS in the hot and humid intrinsic card with immune gene online article20 specificity differences related to the input STRING online analysis,found that one of the 13 genes there exists a close relationship between each other,they form the relationship between the figure,the figure node for 20,to 22.Conclusion:1.The gene chip technology can get HIV/AIDS differences in genetic syndromes.2.Immune genetic variations IL-8,the nf-kappa B,SOD2,TRIB1 express quantity change may be the mechanism of action of immune deficiency causes AIDS people common,immune genetic SOD2 and TRIB1 intrinsic differences between the specificity of gene for damp and hot,may be hot and humid in HIV/AIDS intrinsic mechanism plays a key role.3.Hot and humid embodiment of HIV/AIDS immune related differences exist mRNA interaction relationship network,prompt the gene regulation of each other,common molecular mechanism of hot and humid embodiment in HIV/AIDS syndrome plays an important role.