Research On The Correlation Between Gene Polymorphism Of MMP-1,3 With The Pathogenesis Pelvic Organ Prolapse

Objective:Pelvic organ prolapse(Pelvic organ prolapse,POP)is a disease which is caused by circumstance and genetic factor and mainly occurs in the elderly.But its definite pathogenesis is not clear.POP is not a deadly disease like malignant tumor,but it results in lowerquality of life anddisorders in interpersonal communication.For the past few years,scholars at home and abroad found that the single nucleotide polymorphisms(SNPs)inthepromoterregionsofmatrix metalloproteinase-1(MMP-1)and matrix metalloproteinase-3(MMP-3)can adjust the transcription level of MMP-1 and reduce the protease activity of MMP-3,resulting in decreased extracellular matrix and collagen content,resulting in reduced pelvic floor connective tissue elasticity,finally the weakening of the support for the pelvic floor leads to the occurrence of pelvic organ prolapse.This experiment studied the related susceptibility of single nucleotide polymorphism of MMP-1 and MMP-3 in molecular genetics to pelvic organ prolapse by the molecular biology techniques,and offer new inspiration for early prevention,clinical diagnosis and prognosis.Method:In the research,60 patients with pelvic organ prolapse and60 control groups were enrolled in our hospital.All chosen patients were excluded from suffering from cervical cancer,ovarian tumor,endometrial cancer and other malignant gynecologic disease.Months before the use of hormone therapy or hormone-related diseases,no diseases that related endometriosis,matrix metalloproteinases and matrix metalloproteinase inhibitors.This test researched the gene polymorphisms of the promoter region and the first exon region MMP-1 and MMP-3 in peripheral blood of patients with pelvic organ prolapse and discussed the gene polymorphism of SNP in MMP-1 and MMP-3 and the susceptibility of pelvic organ prolapse with the level of gene.This experiment,with the polymerase chain reaction(PCR)to amplify the target gene fragment and gene sequencing technology through case-control study,detected the genotype frequency distribution and all allele gene frequencyof the SNPs of MMP-1 and MMP-3 in susceptibility gene and used SPSS19.0software to have Chi-square test and to evaluate the risk level of disease.Result:1.The frequencies of 1G and 2G alleles in MMP-1 respectively were 36.67%,63.33%and 57.5%,42.5%,whose distribution has statistical significance(χ~2=10.452,P=0.001).The frequencies of 1G/1G,1G/2G and 2G/2G genotypes in the pelvic organ prolapse group were18.33%,36.67%and 45%respectively.The genotypes of 1G/1G,1G/2G and 2G/2G The frequencies respectively were 41.67%,31.67%and26.66%.The experimental group was different from the control group in statistics(χ~2=8.478,P=0.014).The frequency of homozygous genotype2G homozygote in the experimental group was apparently higher than that in the matched group(χ~2=4.385,P=0.036),The exits of the 2G allele enhance the rate of pelvic organ prolapse,%CI=1.046 to 4.838,OR=2.25 and 95%CI=(1.486 to 7.809),OR=3.406.Compared with the matched group,the MMP-1 was inserted(2G)(P<0.05)the experiment group,which had statistical difference.2.The frequency of 5A and 6A alleles in MMP-3 respectively was 15%,85%and 20.83%,79.17%in PFD group and matched group.There was no statistical difference between the two groups(χ~2=1.388,P=0.239).The frequency of the three genotypes of 5A/5A,5A/6A and 6A/6A in the PFD group were3.34%,23.33%and 73.33%respectively.The frequencies of the three genotypes were 5(8.33%),15(25%)and40(66.67%).There was no significant difference(χ~2=1.511,P=0.47).5A homozygous genotype or5A/6A heterozygous genotype did not improve the risk of pelvic organ prolapse 95%CI=(0.627~3.013),OR=1.375 and 95%CI=(0.396~2.107),OR=0.913.Compared with the matched group,MMP-3was inserted 6A in the experimental group,which had no statistical difference.Conclusion:1.The 1G/2G gene polymorphism of the chimeric region of-1607bp in the promoter region of MMP-1 is related to the pathogenesis of pelvic organ prolapse in women.The 2G allele can obvious enhance the rate of pelvic organ prolapse in women.2.The existence of the 6A allele in the MMP-3 promoter region-1171bp won’t increase the risk of pelvic organ prolapse and cannot concluded the MMP-3 affect the risk of pelvic organ prolapse.