MicroRNA-539 In Hibits Proliferation Of Rheumatoid Arthritis Synoviocytes By Suppressing The Expression Of Osteopontin

Objective: This study was to investigate the role of microRNA-539(miR-539)in the pathogenesis of rheumatoid arthritis(RA)by inhibiting the expression of OPN.Method: A total of 68 patients with RA and 46 patients with osteoarthritis were included in the study.Collect peripheral blood and joint fluid before treatment.The expression levels of osteopontin(OPN)mRNA and miR-539 were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR).The expression of OPN protein was detected by Enzyme-linked immunosorbent assay(ELISA)and Western blotting.Bioinformatics analysis and prediction of the targeting relationship between miR-539 and OPN was validated by dual luciferase assays.In addition,agomiR-539 transfection and siRNA knockdown of OPN were performed in MH7 A cells,and the MTT assay was used to analyze the proliferation of MH7 A cells.Results: The results showed that the OPN was significantly increased in the blood and synovial fluid of RA patients,while the expression of miR-539 was significantly decreased in both samples(P < 0.05).After silencing OPN by siRNA,the proliferation of MH7 A cells decreased(P < 0.05).After upregulation of miR-539,OPN expression was significantly reduced and cell proliferation was inhibited.Dual luciferase assays revealed that miR-539 regulates OPN expression by complementary binding to the 3’ untranslated region.Conclusion: The expression of OPN is significantly increased in the blood and synovial fluid of RA patients,which may be related to the down-regulation of miR-539.Therefore,miR-539 may promote the occurrence and development of RA by regulating OPN.