Construction Of New Anti-tuberculosis Bone Tissue Engineering Three-Dimensional Complex In Vitro

Objective:The objective of the study is to study the construction of a 3D complex by rADSCs,rifapentine polylactic acid sustained-release microspheres and HA/β-TCP,and in vitro construction of a new anti-tuberculous bone tissue engineering complex.Methods:The rifapentine polylactic acid sustained-release microspheres were prepared,whose average diameter was measured,and observed.2D complex of rADSCs-rifapentine polylactic acid sustained-release microspheres was constructed and osteogenic induction was performed,to construct a 3D complex of rADSCs-rifapentine polylactic acid sustained-release microspheres-HA/β-TCP.The adhesion rate was calculated,followed by SEM observation and detection of drug release in vitro.The third-generation rADSCs grew in spindle and triangular shapes.Results:After 14 days of osteogenic induction,they were stained with alizarin,and the appearance of the microspheres was orange-red powder.The microspheres were spherical under electron microscope.No adhesion and agglomeration was formed and the surface was smooth.The particle size of the microspheres was uniform.The cell adhesion rate of rADSCs-HA/β-TCP complex was(94.24±1.25)%,and that of rifapentine polylactic acid sustained-release microsphere-rADSCs-HA/β-TCP complex was(92.02±0.74)%.Drug release of 3D complex continued for up to 46 days,exceeding the minimum inhibitory concentration.Conclusion:The new anti-tuberculous bone tissue engineering has slow and sustained drug release property and osteogenic activity.