Effects Of Salidroside On Proliferation,Apoptosis,Cycle And Migration Of Human Tongue Cancer CAL27 Cells

Objective: 1.To explore the effect of Salidroside on proliferation of CAL27 cells.2.To explore the inducing apoptosis effect of Salidroside on CAL27 cells.3.To explore the effect of Salidroside on cell cycle distribution of CAL27 cells.4.Effect of salidroside on the migration of CAL27 cells.5.Effect of salidroside on the expression of Bcl-2,Bax,VEGF and P56 in CAL27 cells and related mechanisms.Methods: 1.MTT assay was used to evaluate the effect of Salidroside on prolif-eration of CAL27 cells and selected the effect of drug concentration.2.The early apoptosis rate,late apoptosis rate,and necrotic rate of CAL27 cells treated with salidroside were detected by Annexin V/PI double staining and flow cytometry.3.Hoechst33258 fluorescence staining observed CAL27 morphological changes of apoptosis.4.The distribution of CAL27 cells in the treated cells was detected by flow cytometry.5.Transwell chamber experiment was used to observe the effect of salidroside on migration of CAL27 cells.6.Western blot was used to detect the expression of Bcl-2,Bax,VEGF and P56 in CAL27.Results:1.The effect of different concentrations of salidroside(0,20,40,60,80,100μg/mL)on the proliferation of CAL27 cells was detected by MTT.The IC50 at 24 h and 48 h respectively were 133μg/m L/70.1μg/m L,.The inhibition rate of salidroside at the same concentration for 48 h was higher than 24 h;at the same time,the inhibitory rate of the cells was significantly increased with the increase of drug concentration;the above results were statistically significant(p<0.05);The effect of salidroside on proliferation of CAL27 cells was time and concentration dependent.2.Observed by Ho-echst33258 fluorescent staining of cell morphology observed with the salidroside drug concentration increased,the proportion of apoptotic cells also increased,the total number of cells was negatively correlated.After 48 h of salidroside treatment,the results of flow cytometry showed that the proportion of early apoptosis,late apoptosis and total apoptosis of CAL27 cells all increased significantly with the increase of drug concentration,and there was a significant difference in the apoptosis rate of each concentration group.(p<0.05).3.After 48 h of treatment with different concentrations of salidroside on CAL27 cells,the results of flow cytometry showed that the concentration of CAL27 could be changed by each drug concentration,and the proportion of G0/G1 phase increased with increasing drug concentration.Phase and G2 / M phase ratio decreased,indicating that salidroside can make CAL27 cells G0 / G1 phase arrest.4.Transwell chamber experiments showed that with the increase of salidroside drug concentration,the number of cells passing through the basement membrane significantly reduced,and the migration ability of CAL27 cells was significantly affected(p <0.05).5.The results of salidroside on the expression of Bcl-2,Bax,VEGF and P65 protein in CAL27 cells showed that the expression of above proteins was changed after Salidroside of different concentrations were treated with CAL27 cells.With the increase of drug concentration,the expression levels of Bax The expression of Bcl-2,VEGF and P65 decreased(p <0.05).Conclision: Salidroside has effects on the proliferation,apoptosis,cycle and migration of human tongue squamous cell carcinoma CAL27 cells,induces apoptosis of CAL27 cells,induces G0/G1 phase arrest,inhibits migration,and has significant inhibition effects in vitro..