Effect Of Exogenous Hydrogen Sulfide On The Mitochondria Of Epidermal Cells In The Serum Of Burned Rats

Objective:To investigate the effect of exogenous hydrogen sulfide on the mitochondria of epidermal cells in the serum of burned rats.Method:The epidermal cells of the newborn 1 day rat culture in vitro and subculture of adherent cells,the epidermal cells of the third generation were randomly divided into the normal control group,burn group,burn and sodium hydrosulfide group,The changes of mitochondrial membrane potential of epidermal cells were detected after intervention of 3h,12 h and 24 h respectively.Results:(1)At 3 h,12 h,24 h burn group Q2/Q4 ratio compared with normal control group with significant difference(P < 0.05).(2)the ratio of the burn and sodium hydrosulfide group Q2/Q4 in 3h and 12 h was significantly different from that in the control group(P < 0.05).There was no significant difference in the ratio of burn and sodium hydrosulfide group Q2/Q4 at 24 h.(3)the ratio of the burn and sodium hydrosulfide group Q2/Q4 in 3h,12 h and 24 h was significantly different from that in the burn group.Conclusion:Exogenous hydrogen sulfide inhibits the mitochondrial damage of epidermal cells in the serum of burned rats.