The Syndrome Differentiation Of Chronic Pharyngitis And The Pharmacodynamics Of Compound Cumulating Granules

Purpose:By comparing the differences between chronic pharyngitis standard of TCM literature, textbook and clinical TCM literature, reveals the law of syndrome differentiation of chronic pharyngitis. A review of recent 20 years Chinese medicine treatment of chronic pharyngitis reports, proprietary varieties and "database" Puji side "database management system" in diseases related to medication, medication characteristics analysis of chronic pharyngitis. According to the characteristics of traditional Chinese medicine TCM small compound screening from intervention of chronic pharyngitis, and its efficacy is verified by animal experiment. To provide scientific basis for clinical application and drug candidates.Material and method:1.Chronic pharyngitis TCM chaos analysis1.1 Of the "Chinese disease diagnosis and efficacy standards", "guidelines for new drug clinical medicine", "Chinese textbook otolaryngology" and other authoritative literature comparative analysis of the similarities and differences of differentiation standard.1.2 Retrieval(CNKI), Chinese How Net and VIP Wanfang periodical database system traditional Chinese medicine in recent 20 years of literature on the treatment of chronic pharyngitis, summarized statistics of academic degree of dispersion is the main syndrome,such symptoms constitute.1.3 Logical analysis of TCM syndrome differentiation of chronic pharyngitis are fundamental problems, to clarify the ideas and methods of dialectical norms.1.Study on pharmacodynamics of compound granule of increasing liquid particles in the intervention of chronic pharyngitis2.Effect of 2 compound Zengye granule intervention study of chronic pharyngitis2.1 Compound increase liquid particles on xylene induced ear swelling in mice influence:mice 60 were randomly divided into blank group, compound increase liquid particles of low,middle and high dose group, Xuanmaiganjie granule group and DXM group. For each drug0.01ml/g, once a day, continuous administration of gastric administration 5d. Last administered 30 min after, in each mouse left ear ear before and after the two sides withmoving liquid gun uniform coating concentration is 2% of xylene 0.5ml right ear as a control.After half an hour, the neck was put to death, cut off the ears, with a diameter of 8mm punch down the left and right two ear symmetric ear piece weighing, the difference between the two sides of the ear piece weight as a measure of the degree of swelling index. The swelling inhibition rate(%) of the anti- inflammatory effect.2.2 Effects of increasing effects of particles on the agar induced rat granuloma: Taking the weight 150~220g, sixty male and female teenagers were, according to body weight were randomly divided into blank control group, compound increase liquid particles of high,medium and low dose group, Xuanmaiganjie granules group, acetic acid dexamethasone group. In order to prevent the destruction of the agar, for the first time intragastric administration after administration the rats in ether superficial anesthesia under aseptic operation, in the rat dorsal midline subcutaneous injection of 2% agar solution 2ml(pre prepared agar solution, high pressure sterilization for 30min), in accordance with the dose administered intragastrically administered and blank control group were given the same volume of distilled water, once a day, for Administration for 15 days. The 15 th day of anatomy, stripping out granuloma agar block, blot with the filter paper, placed in glass paper use the torsion balance to weigh wet weight, recorded and compared to treatment group and control group, the difference.2.3 Compound increased the influence of liquid particles of chronic pharyngitis in a rat model of: will 60 SD rats were randomly divided into blank control group, model group compound increase liquid particles are low, medium and high dose groups, Xuanmaiganjie granule group,10 rats in each group. Modeling and mouth throat spray into the concentration of 2.5%ammonia, except the blank control group, and eat to need modeling of 50 rats spray, each spray 3 pressed, 1 times a day, continuous spray 16 d. The blank control group with distilled water injection method with the pharynx. The observation index, blood routine examination,pathological observation.Results:1.Chronic pharyngitis and related diseases, the laws of ancient and modern medicine:Modern medicine, houbi with acute pharyngitis and chronic pharyngitis points, correspondingto acute pharyngitis and chronic pharyngitis. But in ancient literature, there is no clear distinction, in conjunction with its match. Ancient treatment of pharyngitis, with licorice root,alum ash, Cimicifuga, Bombyx Batryticatus, Belamcanda chinensis, natural indigo, mirabilite,rhubarb, horse tooth nitrate, borax and natural indigo heat clearing and detoxifying,eliminating phlegm Sanjie drug based and modern use figwort, ophiopogon root, Platycodon root, rehmanniae, Rhizoma Anemarrhenae, Fructus Arctii, Bulbus Fritillariae thunbergii,licorice and other Ziyin reduce pathogenic fire, heat scattered node drug mainly. But the drug specific differences in licorice, others are not the same.2.Chronic pharyngitis syndrome differentiation standard and clinical syndrome differentiation and problems:Chronic pharyngitis syndrome in the literature of the TCM syndrome diagnosis and curative effect standard "" Chinese medicine clinical research guiding principle "and unified teaching materials" Chinese otolaryngology "authority of similarities and differences between each other, merge duplicate, a total of on xuhuo phlogistic card, lung spleen qi deficiency, phlegm heat syndrome, phlegm and blood stasis mutual resistance syndrome, lung dryness due to yin deficiency, lung and stomach heat syndrome, dampness and lung kidney yin deficiency, phlegm and blood stasis syndrome etc.. Generally speaking, TCM etiology and pathogenesis of chronic pharyngitis involves 7 aspects of deficiency, blood stasis, phlegm,gas, heat, poison, wind, viscera positioning involves the lung kidney spleen, liver and stomach.4 aspects of the so-called deficiency including Yin and Yang, Qi and blood deficiency. TCM clinical syndrome differentiation of chronic pharyngitis further discrete, as many as 32 kinds,far beyond the authority of the documents identified by the.3.Compound increase liquid particles xylene induced mouse ear swelling effect: increased compound liquid particles of high, middle dose group and Xuanmaiganjie granule group can inhibited xylene induced ear edema, and blank control group than in the statistical significance(P < 0. 05). The effect of dexamethasone on ear edema induced by xylene was the strongest, and the difference was significant(P < 0.01) compared with the control group.4.Compound increasing effects of particles on the agar induced rat granuloma: increasing compound liquid particles of high,, low dose group, Zhongyang medicine group and Xi Yang medicine group were inhibited growth of agar granuloma in rats, compound increase liquid particles in high dose group, western medicine group and blank there was a significantdifference(P < 0.01) compared with control group, compound increase liquid particles, low dose group, Zhongyang group and blank control group compared had significant difference(P< 0.05).5.Compound increased the influence of liquid particles of chronic pharyngitis in a rat model of: through comparative observation of compound increase particles high dose animals pharyngeal tissue close to normal, only a few animals mucosa gloss poor, chronic mild hyperemia, low secretion. To observe the pathological examination showed that high dose group compared with the model group, the pharyngeal mucosa mild hyperplasia, in the lamina propria of individual existence dilation of blood vessels, significantly reduced the number of inflammatory cells, both in the degree and the number of lesions are compared with the model group, the better recovery. It was confirmed that the high dose group of compound granules had the best effect on the treatment of chronic pharyngitis rats, and the therapeutic effect of the Zhongyang group and the dose group in the compound granules.Conclusion:1. Chronic pharyngitis syndrome differentiation of traditional Chinese medicine should be standardized, should fundamentally solve prevalent among chronic pharyngitis syndrome differentiation of national standards, industry standards and unified teaching materials,academic circles and the standard in chaos. In specific norms, first of all with disease of synonyms and the same syndrome synonym, identify and merge; the necessary choice of the existence of a large number of virtual symptoms and virtual card.2. The ancient and modern treatment of chronic pharyngitis differences larger, as recorded in the ancient literature treating pharyngitis, no acute and chronic, finishing contemporary definitive treatment of chronic pharyngitis literature can confirm from Yin deficiency argument is the emphasis of the syndrome differentiation and treatment. Understanding these differences in adhering to treatment organic combination on the basis of the principle,integrated database and econometric analysis method, optimal screening intervention of chronic pharyngitis curative effect of compound is available for choice of means and path.3.The experimental results show that compound Zengye granule and traditional Chinese medicine positive drug Xuanmaiganjie granule, which can inhibit the mice ear swelling andrat granuloma, but compound Zengye granule and anti-inflammatory effects of high doses of the strongest. The pathological model of chronic pharyngitis pharyngeal tissue of rats, the compound Zengye granule high dose group can obviously reduce epithelial hyperplasia,reduce inflammatory cells of submucosal invasion, reduce vascular dilatation. Conclusion:compound Zengye granule in the treatment of chronic pharyngitis is, can be used as a drug candidate for the follow-up research and development.