The Contrast Between The Two Models Of Subcutaneous Transplantation And Orthotopic Transplantation Of H22 Hepatoma Cells In Mice And The Intervention Study Of Gehua Jieyu Recipe

Objective:1. In BALB/c mice model for the study of liver cancer, compared to mice with H22 hepatoma cells transplanted subcutaneous and orthotopic two kinds of modeling methods.2. Discussion Gehua Jiechen Fang on mice with H22 hepatoma cells transplanted subcutaneous and orthotopic two models, on tumor inhibition rate, immune organ index, tumor necrosis factor-α(TNF-α), superoxide dismutase(SOD) impact indicators. The next step is to choose the best animal model experiments, and further research Gehua Jiechen Fang anticancer mechanism and clinical treatment of liver cancer provide experimental evidence.Methods:1. H22 hepatoma cells establish subcutaneous and orthotopic transplantation model:H22 hepatoma cells established subcutaneous and orthotopic mouse model to observe whether the adhesion of tumor morphology, measuring tumor size, tumor tissue taken HE staining, light microscopy for histological examination. Calculation of tumor formation rate, mortality, immune organ index.2. Gehua Jiechen Fang influence on mice with H22 hepatoma cells in two subcutaneous and orthotopic models:H22 hepatoma cells established subcutaneous and orthotopic mouse model, feeding two weeks after administration were sacrificed blood was collected, remove the liver, thymus, spleen and tumor tissue under the wing. Detection of immune organ index, tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD).Results:1. Two kinds of hepatocellular carcinoma transplantation model modeling tumor rate was 100%. Subcutaneous transplantation mortality rate of 0%,25% mortality of orthotopic transplantation model.2. Both hepatocellular carcinoma transplantation model thymus index were lower (p<0.01 or p<0.05), spleen index increased (p<0.01), but the difference was not statistically significant (p> 0.05) between subcutaneous and orthotopic group. Gehua Jiechen Fang had no significant effect (p>0.05) on the immune organ index two kinds of mice.3. Gehua Jiechen Fang the two hepatocellular carcinoma transplant model has a certain extent, in situ tumor inhibition rate of 53.79%, subcutaneous tumor inhibition rate of 37.05%.4. Gehua Jiechen Fang can significant improvement two kinds of mice TNF-α, SOD (p<0.05 or p<0.01), but the difference was not statistically significant (p>0.05) between the two groups model.Conclusion:1. Aspects of mice with H22 hepatoma cells transplanted subcutaneous and orthotopic model of tumor formation rate, immune organ index were not significantly different.2. Gehua Jiechen Fang of the two liver transplantation model have some inhibitory effect on in situ tumor inhibition rate 53.79% higher than the subcutaneous tumor inhibition rate of 37.05%.3. Gehua Jiechen Fang for the two kinds of mice effect No significant difference (p>0.05), immune organ index, TNF-a, SOD and other indicators of the impact were similar (p> 0.05);4. These conclusions can be drawn between the two kinds of liver transplantation model of Gehua Jiechen Fang reaction was no significant difference, subcutaneous transplantation model easier than orthotopic model, it is more suitable for subcutaneous transplantation model Gehua Jiechen Fang effects on liver cancer in mice research.