A Comparative Study On The Drying And Drying Of Rice Bran Water

Purpose: To concocted Belamcanda chinensis(L.)DC by Using the method from Ancient.And make them extract of the sample before and after.From the chemical composition,anti-inflammatory pharmacodynamics,in vivo pharmacokinetics of the two compared,It is expected to find the significance of this method,and then to improve the clinical efficacy of the proposed new method.Method:1.First,according to the ancient literature and modern Chinese herbal medicine processing standards for the dry rice swill processing,and then AB-8 macroporous adsorption resin column purification of raw materials and products prepared into the corresponding extract.The comparison of the content of the known components before and after the preparation and the relative content of the isoflavones was compared with the conventional method of external standard method and the multi-evaluation method.To find the change of chemical composition of rice swill.Comparative Study on Variation of Chemical Components in Processing Products and Raw Products.2.Based on the carrageenan-induced inflammation model,the anti-inflammatory pharmacodynamics of the shoots were compared.The toe volume was measured by the toe volume measuring instrument before and after inflammation,and the toe swelling rate and inhibition rate were calculated.The anti-inflammatory effect of the rats before and after the preparation was evaluated by comparing the inhibitory rate of swelling in each group.The content of arachidonic acid in the inflammatory index was evaluated by LC-MS / MS.in vivo inflammation index Changes in the situation.3.The pharmacokinetic studies of the related substances in Beagle dogs were compared by oral administration.The pharmacokinetic characteristics of dried shoots were studied.The plasma concentration of iris flavin and wild iris were determined by LC-MS / MS,and the plasma concentration-time curve was obtained according to these data.The results of room model t1 / 2α,T1 / 2β,AUC(0-t),etc.,the statistical parameters such as MRT,Tmax,Cmax and other pharmacokinetic parameters were compared with the pharmacokinetic parameters of the product and the product.The change of the method and the characteristics of the change before and after the processing.Results:1.The results showed that the content of mangiferin,Radix glycosides and Rattan was reduced by 56.20%,and the contents of mangiferin 80.05% and 79.01%,respectively.The contents of Isoflavonoids,Iris flavonoids,Iris A,Iris flavonoids B,irigenin,Irisflorentin,dichotomitin,The average content of unknown isoflavones was decreased by 75.42%,230.32%,173.88%,120.76%,12.90% and 11.98%,respectively.The unknown components Ⅰ and Ⅱ were75.74% and 66.46% respectively.2.Through the comparative study on the antiinflammatory effect of inflammation model of rat toe swelling,it was found that the anti-inflammatory effect of the dried skin was improved.This may be due to the processing of the dry anti-inflammatory ingredients in the content of iris flavors increased,thereby enhancing the anti-inflammatory effect of drugs.Although the literature reported that the dry shoots in the body will be in its body to its aglycone(irretic flavin)conversion,and then play an anti-inflammatory effect,but the results from the test to see the effect of this in vivo transformation is not as good as in vitro processing effect is ideal,also shows The results showed that the content of glycoside was increased and the anti-inflammatory effect was improved.There was no significant difference in the content of arachidonic acid in the inflammatory metabolic intermediates of different administration groups.Suggesting that the role of anti-inflammatory anti-inflammatory pathway may be completed by the downstream products of arachidonic acid.3.The metabolic characteristics of tectorigenin and irigenin were changed in the study of pharmacokinetic parameters of active ingredients in rabbits before and after processing.This change indicates that the time of distribution of tectorigenin and irigenin in the dried shoots is shortened,and the glycosides are directly converted into aglycones after in vitro treatment.After oral administration,tectorigenin and irigenin were more rapidly absorbed than raw products,and the AUC of tectorigenin was significantly increased,and the product MRT was also prolonged with the product,indicating that the processing was not only faster,but also bioavailability High,drug retention time is also slightly extended.Since the chemical properties of iris flavonin did not change,there was no change in Tmax before and after composting,but Cmax was significantly increased and peaked at high concentration,which may be the reason for the increase in anti-inflammatory effect after shaking.The AUC of the genus Iris was almost unchanged before and after the treatment,and the mean retention time MRT was also different,but Tmax was slightly shortened and Cmax was increased.After the combination(raw goods and products),the serum chromatogram Will appear in the form of bimodal,consider it did not reach the increase in the proportion of in vitro,the reason may be wild iris in the body will be converted into isomers to play a therapeutic role,for this point is expected to further study.Conclusion:1.The results showed that there was no new component in the processing of the dried water after the processing of the rice swill.The content of the known glycosides(Mangoside,acylate and wild irisin)was(tectorigenin、Iristectorigenin A、Iristectorigenin B、irigenin、Irisflorentin、dichotomitin)increased;unknown isoflavones compounds Ⅰ,Ⅱ The content also decreased.As a whole to prove that the meter swamp will make the shoots of the glycosides in the composition of glycosides into components.2.The effect of anti-inflammatory effect after weaning was improved,and the content of arachidonic acid in inflammatory cells did not change significantly.Suggesting that the role of anti-inflammatory anti-inflammatory pathway may be completed by the downstream products of arachidonic acid.3.The results showed that the pharmacokinetic characteristics of tectorigenin and irigenin were changed,and the metabolic behavior of iris flavonoids was significantly lower than that of the control group Have an impact.In general,the meter swamp can reduce the content of glycosides and increase the content of aglycones.This change can lead to the change of pharmacokinetic characteristics in vivo,and then improve the anti-inflammatory effect.